Báo cáo khoa học: "Does Doxycycline work in synergy with cisplatin and oxaliplatin in colorectal cancer?" ppt

... levels in cells treated with cisplatin and oxaliplatin in combination with doxy- cycline (p = 0.001 and p = 0.039 respectively). These find- ings corresponded with the findings obtained in caspase ... lines [13,14]. In this study we investigated whether doxycycline works in synergy with cisplatin and oxaliplatin or not in HT 29 colorectal cancer cell li...

Ngày tải lên: 09/08/2014, 04:20

... disease in 42% of cases, up regulating in 28% and down regulating in 14% of cases [2]. The procedure con- sisted of liver and lymph node biopsies together with splenectomy. In addition to allowing ... 5); inadequate (n = 4). The remaining 30 patients proceeded to biopsy without FNAC. FNAC was performed in 18 of 23 patients with cervical lymphaden- opathy but in only 14 of...

Ngày tải lên: 09/08/2014, 07:21

... data in yeast suggest that the Alr1 protein acts as a Mg 2+ -permeable ion channel [14]. Using in vitro chemical cross-linking and in vivo split-ubiquitin assays to analyze protein–protein inter- actions, ... overexpressing an Alr1-HA fusion protein, followed by SDS ⁄ PAGE and immuno- blotting to detect Alr1p-containing products (Fig. 7). Without the cross-linking agents, Alr1p was...

Ngày tải lên: 19/02/2014, 06:20

... interactions between FadD29 and PKS15 ⁄ 1 on the one hand, and FadD26 and the FasI system on the other hand. Further inves- tigations, including protein interaction studies and structural studies, ... with [1- 14 C]propionate, a precursor known to be incorpo- rated into methyl-branched fatty acyl-containing lipids, including DIMs. Lipids were then extracted from the recombinant stra...

Ngày tải lên: 15/03/2014, 11:20

... selenium compound in 100 lLof DMEM containing 2% fetal bovine serum at 37 °Cinan atmosphere of 95% air and 5% CO 2 . PMNs (2 · 10 4 ) were added to a 96-well microplate and were incubated with 100 lm ... culture and cytotoxicity assay HaCaT cells, HEL cells and HEp-2 cells were cultured in DMEM supplemented with 10% fetal bovine serum, l-gluta- mine (0.6 mgÆmL )1 ) and 0.35%...

Ngày tải lên: 23/03/2014, 07:20

... were treatedwithNaCl/P i containing 3% h ydrogen peroxide and 10% methanol for 15 min; nonspecific binding with PKCa and PKCe was blocked by incubation for 30 min with NaCl/P i containing 1% BSA. ... suggestio n is consistent with data in the literature that indicate PKCe as the only protein kinase isoform involved in the s ignalling pathway downstream of muscarinic m3 receptors...

Ngày tải lên: 23/03/2014, 13:20

... Engineering and Electrical Engineering University of Washington Seattle, WA 98195, USA {karim@cs,bilmes@ee}.washington.edu Abstract Sitting at the intersection between statis- tics and machine ... of finding embeddings in a k-tree. SIAM J. Algebraic Discrete Methods, 8(2):277–284. L. R. Bahl and F. Jelinek. 1975. Decoding for channels with insertions, deletions, and substitutions...

Ngày tải lên: 23/03/2014, 19:20

... structure, retains flavin binding, and shows an increased sensitivity to trypsin of a specific site (Arg289) belonging to the loop preceding the b-strand F5 of the FAD-binding domain. Strand F5 has ... determined by titrating the apoprotein with increasing amounts of FAD and following the decrease in protein fluorescence: a K d value of 0.37 and 0.035 lm of the apoprotein–FAD complex w...

Ngày tải lên: 30/03/2014, 11:20

... D.S. (1999) Interactions controlling the membrane binding of basic protein domains: phenylalanine and the attachment of the myristoylated alanine-rich C-kinase substrate protein to interfaces. ... poneratoxin and pon- eratoxin obtained by expression in insect cells. When expressed in insect cells, poneratoxin was observed attached to cell membranes. Both synthetic and recom- binant...

Ngày tải lên: 30/03/2014, 13:20

... neurons against apoptosis induced by DNA damage [15,16]. In these studies, DNA damage was induced by genotoxic anticancer drugs, camptothecin or cisplatin (CPDD). Interestingly, cisplatin is highly ... signaling ERK1,alsoknownasp44mitogenactivatedproteinkinase (MAP kinase), and ERK2 (p42 MAP kinase) are closely related protein kinases of the MAP kinase family [3]. ERK1/2 is regulated...

Ngày tải lên: 30/03/2014, 14:20