Báo cáo y học: "Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant" pdf

... Research article Highly efficient genetic transduction of primary human synoviocytes with concentrated retroviral supernatant Jianmin Yang, Michael S Friedman, Huimin Bian, Leslie J Crofford, Blake Roessler ... g, with complete recovery of infectious viral parti- cles. This data is presented in the Supplementary Material (Supplementary Figs 1, 2, 3, and 4). Retroviral...

Ngày tải lên: 09/08/2014, 03:24

... Belozersky Institute of Physico-Chemical Biology, Moscow State University; and 2 A. N. Bach Institute of Biochemistry, Russian Academy of Sciences, Moscow, Russia The thermal unfolding of myosin ... actin-binding sites. Keywords: myosin subfragment 1; thermal unfolding; differential scanning calorimetry. Cyclic association–dissociation of actin and myosin coupled with ATP hydrolysi...

Ngày tải lên: 17/03/2014, 10:20

... triphosphate. Synthesis, properties, and interaction with myosin and heavy meromyosin. Biochemistry 11, 2863–2871. 34. Pfeuffer, T. & Eckstein, F. (1976) Topology of the GTP-binding site of adenylyl cyclase ... stable with up to 190-fold lower hydrolysis rates were the two triphosphate analogues with b,c-substitutions GppCH 2 p and GppNHp. The Ras-catalysed hydrolysis rate of G...

Ngày tải lên: 18/03/2014, 01:20

... two experiments, one of which is given in F ig. 2) were 7 days using D eM, 2 6 days using UM and 40± 130 days using DDM. When CHS was employed in combination with any of the three alkylm altosides, ... structure determination of hA2aR by X-ray or electron crystallography, or infor- mation on the bound ligand conformation obtained by NMR spectroscopy, would assist in the design of su...

Ngày tải lên: 24/03/2014, 00:21

... major secretory protein of FLS cells, macrophages and chondrocytes [21]. Although HC gp-39 displays structural homology with members of the Family 18 chitinases it lacks enzymatic activity, raising ... GS, Li D, Leiper JM, Siragy HM, Carey RM, Johns RA: Evidence for dysregulation of dimethylarginine dimethylaminohydrolase I in chronic hypoxia-induced pul- monary hypertension. Circulat...

Ngày tải lên: 09/08/2014, 01:23

... resem- bles that of the chondrocytes of articular cartilage [1], they produce predominantly type I collagen with smaller amounts of types II, III, V and VI collagens, and small amounts of aggrecan [13]. ... the effect of low oxygen tension, which has previously been shown to enhance matrix assembly by chondrocytes, was investigated. Effect of FGF2 expansion on chondrogenic respo...

Ngày tải lên: 09/08/2014, 07:20

... citation purposes) lymphocytes. Several subsets of CD4+ cells with immuno-reg- ulatory activity have been described, including T regulatory (T REG ) lymphocytes, and type-1 T regulatory (Tr1) cells ... citation purposes) flow cytometry analysis. Finally, Ts lymphocytes (CD8+CD28) were also detected by two-color flow cytometry analysis using specific mAbs. Detection of apoptosis Fresh is...

Ngày tải lên: 09/08/2014, 08:22

... modifications. Keywords: Coronary artery bypass graft surgery, CABG, Off-pump surgery, OPCAB, Pectus excavatum, Poland Syndrome Off-Pump Coronary Artery Bypass in Poland syndrome with dextrocardia: ... successfully underwent off-pump coronary artery bypass grafting (OPCABG). Case Summary A 66-year-old male presented with a two-month history ofCCSclassIIangina.Hehadahistoryoftwofailed repa...

Ngày tải lên: 10/08/2014, 09:21

... Touraine R: Primary pulmonary pseudolymphomas and lymphomas.4 cases. Rev Mal Respir 1984, 1(2):105-11. 35. Watanabe J, Yamaguchi K, Sugiyama Y, Yotsumoto H, Takaku F: Primary pulmonary lymphoma demonstration ... T, Akiyama J, Yamakawa H, Ikehara K, Ito M, Fujii M, Suzuki M, Arai K: Primary pulmonary lymphoma diagnosed from monoclonality of lymphocytes in a transbronchial biopsy speci...

Ngày tải lên: 10/08/2014, 09:23

... washed, labelled with an anti-CD8 antibody and analysed by flow cytometry. The percentage of dividing T-cells was linearly correlated with the loss in CFSE fluorescence. Flow cytometric analysis The ... con- centration of 2–5 × 10 6 of cells per 200–500 μl of medium. After 3 hours at 37°C, cells were placed in com- plete medium and analysed by flow cytometry between day 5 and day...

Ngày tải lên: 18/06/2014, 15:20