... TRAF1 were designated HeLaTR/ 4-1 BB and HeaLaTR/ TRAF1, respectively. Primers for amplifying the 4-1 BB and TRAF1 cDNAs were: 5¢-GAATTCAAGCTTATGGGA AACAGCTGTTACAACATA-3¢ and 5¢-GAATTCAAG CTTCACAGTTCACATCCTCCTTCTTCT-3¢ ... 5¢-GAATTCAAG CTTCACAGTTCACATCCTCCTTCTTCT-3¢ for 4-1 BB, 5¢-CCCGGGATATCATGGCCTCCAGCTCAGGCAG CAGTC-3¢ and 5¢-CCCGGGATATCTAAGTGCTGG TCTCCACAATGCACT-3¢ for TRAF1....
Ngày tải lên: 23/03/2014, 18:20
... of chemical cleavage at aspartate residues. We were aware of the possible occurrence of deamidations of asparagines and subsequent partial cleavage at the resulting aspartate residues. However, virtualmslab analysis ... moderate number of alternative candidates should still allow unambiguous identifica- tion based on additional tandem mass spectrometric validation. It thus appear...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Frataxin deficiency causes upregulation of mitochondrial Lon and ClpP proteases and severe loss of mitochondrial Fe–S proteins pot
... Sciences, Meylan, France). Lon protease (forward, 5¢-AGGATCTTGCCTTGTGT GGA-3¢; reverse, 5¢-TGGATGAGGAGCTGAGCAAG-3¢) ClpP (forward, 5¢-CACACCAAGCAGAGCCTACA-3¢; reverse, 5¢-CCCAGCAGAGGAAGTTTCAG-3¢), mitochon- drial ... 5¢-GGAGGGCAAGCAACAGTATC-3¢; reverse, 5¢-GCGTTCCTCTGTGAAGTCGT-3¢), Rieske (forward, 5¢-TGGTCTCCCAGTTTGTTTCC-3¢; reverse, 5¢-GCAGC TTCCTGGTCAATCTC-3¢) and SDHA (forward, 5¢-CAG A...
Ngày tải lên: 23/03/2014, 06:20
Tài liệu Báo cáo khoa học: Differentiation stage-dependent preferred uptake of basolateral (systemic) glutamine into Caco-2 cells results in its accumulation in proteins with a role in cell–cell interaction pptx
... 5.2 ± 0.3 for 5-day- and 15-day-differentiated cells, respectively. At 24 h the basolateral ⁄ apical uptake ratio was 3.0 ± 0.6 and 1.7 ± 0.3 for 5-day and 15-day-differentiated cells, respectively. ... functional groups. The first group of proteins (annexin A2 , annexin A4 , cad- herin-17, galectin-3 and alpha-actinin 4) is involved in membrane stabilization, cell cell adhesion...
Ngày tải lên: 20/02/2014, 01:20
Tài liệu Báo cáo khoa học: Oxygen tension regulates the expression of a group of procollagen hydroxylases docx
... I Sense: 5¢-CGGGATCCTCGGACACCCTGTAAATG-3¢ Antisense: 5¢-GGAATTCCAAGCAGTCCTCAGCTGT-3¢ Mouse (gi:6754969) prolylhydroxylase alpha II Sense: 5¢-CGGGATCCTGCAGGCAGAATTCTTCA-3¢ Antisense: 5¢-GGAATTCCCAGTCTGTGTTCAACCG-3¢ Rat ... lysylhydroxylases mRNAs. Mouse (gi:836897) prolylhydroxylase alpha I Sense: 5¢-CGGGATCCTAGACCGGCTAACAAGTA-3¢ Antisense: 5¢-GGAATTCCAAGCAGTCCTCAGCTGT-3¢ Rat (gi:474939) proly...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Post-ischemic brain damage: targeting PARP-1 within the ischemic neurovascular units as a realistic avenue to stroke treatment pptx
... triggers DNA damage-dependent hyperactivation of PARP-1, depletion of intracellular NAD and ATP stores, and neuronal death [26]. PARP-1 activation may also occur in neurons without NMDA receptor activation, ... result of the fact that inflammatory transcription factors such as nuclear factor-kappaB, activator protein-1 and nuclear factor of activated T-cells are positively regula...
Ngày tải lên: 07/03/2014, 03:20
Báo cáo khoa học: Epl1, the major secreted protein of Hypocrea atroviridis on glucose, is a member of a strongly conserved protein family comprising plant defense response elicitors potx
... [e.g. cerato-platanin of Ceratocystis fimbriata f. sp. platani, Snodprot1 of Phaeosphaeria nodorum and Sp1 of Leptosphaeria maculans) or human allergens and path- ogenesis-related proteins (As-CG of Coccidioides ... detected, and by far the most abundant spot (g1 in Fig. 1) was a small protein (approximately 16 kDa, pI 5.5–5.7), and this was followed by spot g2, with a...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: The natural mutation by deletion of Lys9 in the thrombin A-chain affects the pKa value of catalytic residues, the overall enzyme’s stability and conformational transitions linked to Na+ binding pdf
... thrombin, the A- chain assumes an overall boomerang-like shape interacting with the B-chain sur- face opposite to the active site [17]. Stabilization within the A- chain and between the A- and B-chains ... report, DK9 compared with WT thrombin achieves a better interaction with the low-molecular mass inhibitor N -a- (2-naphthylsulfonyl- glycyl )-4 -amidinophenylalanine-piper...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx
... high- potential and a low-potential class. The high-potential class contains [4Fe-4S] and [3Fe-4S] clusters with redox potential of +180 mV and +130 mV, respectively. Redox potentials of )55 and )420 mV ... time constants corresponding to apparent rate constants are obtained by fitting Eqn (1): DAbs ¼ a þ b e Àct ð1Þ where, DAbs is the variation of absorbance, a and b a...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Unravelling the functional interaction structure of a cellular network from temporal slope information of experimental data docx
... cerevisiae MAP kinase cascades typically composed of three tiers of protein kinases, a MAP kinase (MAPK), a MAPK kinase (MAPKK) and a MAPKK kinase (MAPKKK), are common signalling modules in eukaryotic cells ... that can be applicable to time-series experimental data without parameter perturbations and that can make use only of the qualitative information without relying on mea...
Ngày tải lên: 07/03/2014, 21:20