... residual cellulose wassubsequently washed with 50 and 12.5 mm Na2HPO4/NaH2PO4(pH 7.0). The cellulosome-containing fractionwas eluted from the residual cellulose with water, dialysed and ... with cellobi-ose (4 gÆL)1; Sigma-Aldrich, St Louis, MO, USA) orMN300 cellulose (5 gÆL)1; Serva, Heidelberg, Germany).Purification of the cellulose-adsorbed cellulolyticsystem from C. cellulolyticumC. ... widespread thanexpected and have a common and important functionin cellulosome regulation, displacement from the cell surface, degradation and ⁄ or protection of the cellulo-somes [40].All...