Báo cáo khoa học: "Aeration of the root system in Alnus glutinosa L Gaertn P Schröder" doc

Báo cáo khoa học: "Aeration of the root system in Alnus glutinosa L. Gaertn. P. Schröder" doc

... the atmosphere and the intercellular system inside. Intercellularspaces 1-5 pm in diameter are frequentlyfound in the lenticel tissue of young leaf-less alders (K6stler ... may,according to the theory, be due to the existence of small intercellular spacesinside the lenticel tissue stimulating ther-mo-osmosis of gases. In A. incana, mea-surements ... Die INurzeln der Waldbiume. PareyPubl., HamburgSchr6der P. (1986) Thermo-osmotischersauerstofftransp!ort in Ainus glutinosa undNuphar lutea als anpassung an ein leben in anaerober...

Báo cáo khoa học: Characterization of the serotoninergic system in the C57BL/6 mouse skin potx

... analysis by the ampliﬁcation of housekeeping gene GAPDH as describedpreviously [25]. Mouse TPH was ampliﬁed by a single PCR,while serotonin AANAT was ampliﬁed by nested PCR. The localization of ... Memphis, TN, USA;4Department of Internal Medicine, Southern Illinois University, Springﬁeld, IL, USAWe showed expression of the tryptophan hydroxylase geneand of tryptophan hydroxylase protein ... all samples of mouse corporal skin, although it was detectable at low levels in the ears and in Cloudman melanoma (derived from the DBA/2 J mouse strain). In conclusion, mouse skin has the molecular...

Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx

... superfamily play import-ant roles in the inhibition of serine proteases involved in complex systems.This is evident in the regulation of coagulation serine proteases, especially the central ... (RCL) of the serpin [5]. The catalysis of the peptide bond cleavage appears to be arrested at the acyl intermediate by the unique action of the serpin,whereby the RCL of the serpin inserts into ... of another molecule’s RCL takes place [8]. Both of theseresult in the irreversible inactivation (generally) of the serpins, and, in the case of the anticoagulant serpins, alowering of the effective...

Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf

... identiﬁed the pres-ence of P- LAP protein in the renal distal tubules and col-lecting ducts. In rat NRK52E cells derived from renaltubules, P- LAP was localized mainly in the intracellularcompartment. ... placental leucineaminopeptidase; vasopressin. The serum level of placental leucine aminopeptidase (P- LAP)/oxytocinase increases during pregnancy [1]. It isgenerally believed that P- LAP plays an important ... cell lines.It is well known that AVP regulates water reabsorption in renal collecting duct principal cells by activating the cAMP-dependent translocation of AQP-2 from an intracellularcompartment...

Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot

... chimeric proteinAll the chimeric proteins were puriﬁed following the one-step procedure reported previously for the puriﬁcation of horse recombinant PLRP2 expressed in insect cells [8]. The culture ... composed of a PL domain and a PLRP2 domain are described in Fig. 1. First, pVLhoPL, previously described, resulted in the integration of the nucleotide sequence encoding hoPL,including the peptide ... al. [35]. Indeed, theseauthors showed that, in the absence of bile salts, achimeric protein composed of the N-terminal domain of guinea pig PLRP2 and of the C-terminal domain of human PL (gpN2...

Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot

... the isoleucine residue is the most important:even its replacement by a very closely related aminoacid, leucine, substantially impairs the intracellularphosphorylation of 4E-BP1. Proline also ... The level of phosphorylation of Ser64 in insulin-treated cells is therefore especially informative.We have shown previously that mutation of Phe113to alanine in the 4E-BP1 TOS motif markedly ... raptor (although it is not essential for this),and that other regions of the 4E-BP1 polypeptide areinvolved in this interaction (especially parts of the C-terminal half of the molecule). The present...

Báo cáo khoa học: Characterization of the ﬂavin association in hexose oxidase from Chondrus crispus pot

... and0.5 mL of 6 m HCl was added to the lyophilized protein in sealed glass vials. The vials were incubated in the dark for16 h at 95 °C and lyophilized. Samples were resuspended in 1 mL of deionized ... determination of the peptide–ﬂavin complexwas carried out simultaneously with the chromatogra-phy steps using an LC-MS ⁄ MS setup (see Experimen-tal procedures). Flavopeptide-related ions in the ... Determining the mass of the pep-tide–ﬂavin complex isolated from a hexoseoxidase (HOX) trypsin digest by RP-HPLC.(A) The LC-MS chromatogram of a trypsindigest of HOX. The absorbance was con-tinually...

Báo cáo khoa học: Modulation of the endocannabinoid system by focal brain ischemia in the rat is involved in neuroprotection afforded by 17b-estradiol pdf

... hydrolase; MCA, middle cerebral artery; MCAo, middle cerebral artery occlusion; NAPE, N-acylphosphatidyl-ethanolamine; NAPE-PLD,N-acylphosphatidylethanolamine-hydrolyzing phospholipase D; NArPE, ... stages of reperfusion in the ischemic striatum. Striatal downregulation of FAAHand upregulation of NAPE-PLD activity lead toincreased levels of AEA, which in turn may play a role in the pathophysiology ... into the internal carotid artery, approximately18 mm from the carotid bifurcation, until mild resistancewas felt, thereby indicating occlusion of the origin of the MCA in the Willis circle. The...

Báo cáo khoa học: Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase pptx

... subsequently analyzed in six wells, each in a thin-walled 96-well PCR plate in 50 lL aliquots per well. The real-time PCR machine used was an i-Cycler (Bio-Rad,Hercules, CA, USA) providing a MyiQ Optics ... on lipophilic substrates, and the open form of the loop interacts with the hydrophobic part of choles-terol and is thus responsible for substrate speciﬁcity[34]. Similarly, the loop of P2 O in ... Crystallogr D Biol Crystallogr 50, 178–185.Supporting information The following supplementary material is available:Table S1. Nucleotide sequences of synthetic oligonucle-otides used for the P2 O...

báo cáo khoa học: " Activation of the hedgehog pathway in chronic myelogeneous leukemia patients" ppt

... (Smo), and Gli1 genes of Hh signaling weresignificantly upregulated in CML patients when compared with normal people (P < 0.001). The levels of Shh, SmomRNA in chronic phase of CML patients ... receptors in CML patients and normal control. Lane 1:normal control 1:Lane 2:normal control 2:Lane 3:CML-CP case 1:Lane 4:CML-CP case 2:Lane 5:CML-AP case 1:Lane 6:CML-AP case 2:Lane7:CML-BC case 1:Lane8: ... resulting in the for-mation of Philadelphia(Ph) chromosome and at the molecular level, a chimeric gene known as BCR-ABLresponsible for CML initiation. CML often initiates in achronic phase,...

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