... the atmosphere and the intercellular system inside. Intercellular spaces 1-5 pm in diameter are frequently found in the lenticel tissue of young leaf- less alders (K6stler ... may, according to the theory, be due to the existence of small intercellular spaces inside the lenticel tissue stimulating ther- mo-osmosis of gases. In A. incan...
Ngày tải lên: 09/08/2014, 02:21
... analysis by the amplification of housekeeping gene GAPDH as described previously [25]. Mouse TPH was amplified by a single PCR, while serotonin AANAT was amplified by nested PCR. The localization of ... Memphis, TN, USA; 4 Department of Internal Medicine, Southern Illinois University, Springfield, IL, USA We showed expression of the tryptophan hydroxylase gene and of tryptophan hy...
Ngày tải lên: 31/03/2014, 07:20
Tài liệu Báo cáo khoa học: Control of the coagulation system by serpins Getting by with a little help from glycosaminoglycans pptx
... superfamily play import- ant roles in the inhibition of serine proteases involved in complex systems. This is evident in the regulation of coagulation serine proteases, especially the central ... (RCL) of the serpin [5]. The catalysis of the peptide bond cleavage appears to be arrested at the acyl intermediate by the unique action of the serpin, whereby the...
Ngày tải lên: 20/02/2014, 02:21
Báo cáo khoa học: Involvement of the V2 receptor in vasopressin-stimulated translocation of placental leucine aminopeptidase/oxytocinase in renal cells pdf
... identified the pres- ence of P- LAP protein in the renal distal tubules and col- lecting ducts. In rat NRK52E cells derived from renal tubules, P- LAP was localized mainly in the intracellular compartment. ... placental leucine aminopeptidase; vasopressin. The serum level of placental leucine aminopeptidase (P- LAP)/oxytocinase increases during pregnancy [1]. It is general...
Ngày tải lên: 08/03/2014, 02:20
Báo cáo khoa học: Role of the structural domains in the functional properties of pancreatic lipase-related protein 2 pot
... chimeric protein All the chimeric proteins were purified following the one- step procedure reported previously for the purification of horse recombinant PLRP2 expressed in insect cells [8]. The culture ... composed of a PL domain and a PLRP2 domain are described in Fig. 1. First, pVLhoPL, previously described, resulted in the integration of the nucleotide sequence encodi...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Analysis of the regulatory motifs in eukaryotic initiation factor 4E-binding protein 1 pot
... the isoleucine residue is the most important: even its replacement by a very closely related amino acid, leucine, substantially impairs the intracellular phosphorylation of 4E-BP1. Proline also ... The level of phosphorylation of Ser64 in insulin-treated cells is therefore especially informative. We have shown previously that mutation of Phe113 to alanine in the 4E-BP1 T...
Ngày tải lên: 16/03/2014, 06:20
Báo cáo khoa học: Characterization of the flavin association in hexose oxidase from Chondrus crispus pot
... and 0.5 mL of 6 m HCl was added to the lyophilized protein in sealed glass vials. The vials were incubated in the dark for 16 h at 95 °C and lyophilized. Samples were resuspended in 1 mL of deionized ... determination of the peptide–flavin complex was carried out simultaneously with the chromatogra- phy steps using an LC-MS ⁄ MS setup (see Experimen- tal procedures). Flav...
Ngày tải lên: 16/03/2014, 14:20
Báo cáo khoa học: Modulation of the endocannabinoid system by focal brain ischemia in the rat is involved in neuroprotection afforded by 17b-estradiol pdf
... hydrolase; MCA, middle cerebral artery; MCAo, middle cerebral artery occlusion; NAPE, N-acylphosphatidyl- ethanolamine; NAPE-PLD,N-acylphosphatidylethanolamine-hydrolyzing phospholipase D; NArPE, ... stages of reperfusion in the ischemic striatum. Striatal downregulation of FAAH and upregulation of NAPE-PLD activity lead to increased levels of AEA, which in turn may play a role...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: Importance of the gating segment in the substrate-recognition loop of pyranose 2-oxidase pptx
... subsequently analyzed in six wells, each in a thin-walled 96-well PCR plate in 50 lL aliquots per well. The real-time PCR machine used was an i-Cycler (Bio-Rad, Hercules, CA, USA) providing a MyiQ Optics ... on lipophilic substrates, and the open form of the loop interacts with the hydrophobic part of choles- terol and is thus responsible for substrate specificity [34]. Simil...
Ngày tải lên: 29/03/2014, 09:20
báo cáo khoa học: " Activation of the hedgehog pathway in chronic myelogeneous leukemia patients" ppt
... (Smo), and Gli1 genes of Hh signaling were significantly upregulated in CML patients when compared with normal people (P < 0.001). The levels of Shh, Smo mRNA in chronic phase of CML patients ... receptors in CML patients and normal control. Lane 1:normal control 1:Lane 2:normal control 2:Lane 3: CML-CP case 1:Lane 4:CML-CP case 2:Lane 5:CML-AP case 1:Lane 6:CML-AP case 2:La...
Ngày tải lên: 10/08/2014, 10:20