Báo cáo khoa học: "Dormancy and spring burst of lateral low-pruned mulberry (Morus alba L )" pdf

Báo cáo khoa học: Protein and mRNA content of TcDHH1-containing mRNPs in Trypanosoma cruzi pdf

... (2007) Accumulation of polyadenylated mRNA, Pab1p, eIF4E, and eIF4G withP-bodies in Saccharomyces cerevisiae. Mol Biol Cell 18,2592–2602.34 Hoyle NP, Castelli LM, Campbell SG, Holmes LEA &Ashe ... Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granulesthat are kinetically and spatially distinct from P-bodies.J Cell Biol 179, 65–74.35 Buscaglia CA, Campo VA, ... and all lanes were loaded with 10 lgofprotein. (B) SDS ⁄ PAGE stained with Coomassie brilliant blue tocontrol protein load. TcDHH1 has an expected molecular mass of 46.7 kDa. The molecular mass...

Tài liệu Báo cáo khoa học: Functional and structural analyses of N-acylsulfonamidelinked dinucleoside inhibitors of RNase A ppt

... structural complexes of two N-acylsulfonamide-linked nucleosides withRNase A closely mimic the binding by nucleic acids.The attributes and versatility of N-acylsulfonamidyl and sulfonimidyl groups ... informationThe following supplementary material is available:Fig. S1. Atom numbering for compounds 6 and 7.Table S1. Torsion angles of nucleosides in RNase AÆN-acylsulfonamidelinked nucleoside complexes.Table ... detail by using X-ray crystallography.Three-dimensional structures of RNase AÆN-acyl-sulfonamide-linked nucleoside complexesThe three-dimensional structures of N-acylsulfona-mides 6 and 7...

Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc

... that is correctly folded and a largeamount of incorrectly folded and insoluble protein. Itis conceivable that a cN-II protein that is incorrectlyfolded but still soluble is also produced. A ... dell’Istruzione, dell’Universita`e dellaRicerca and by local funds from the University of Pisa.References1 Tozzi MG, Camici M, Pesi R, Allegrini S, Sgarrella F& Ipata PL (1991) Nucleoside ... members of the haloacid dehalogenase (HAD) superfamily,including the soluble 5¢-nucleotidase family: cytosolic5¢-nucleotidases cN-II, cN-III, cN-IA and cN-IB, and both cytosolic and mitochondrial...

Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx

... proteincrystallography. Acta Crystallogr D Biol Crystallogr 50,760–763.31 Vagin A & Teplyakov A (2000) An approach to multi-copy search in molecular replacement. Acta CrystallogrD Biol Crystallogr ... coliBL21(DE3) strain and stored at )80 °C for further use.One hundred and ﬁfty milliliters of LB supplemented with50 lgÆmL)1kanamycin was inoculated and grown at 37 °Covernight; 40 mL of ... this culture were used to inoculate 3 L of TB supplemented with 50 lgÆmL)1kanamycin and approximately 50 lL of Breox antifoam (Cognis Perfor-mance Chemical UK Ltd) in glass ﬂasks using the largescale...

Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx

... whole-cell conversion of d ,l- p-HPH was alsoWTF63CF63DF63EF63GF63HF63IF63KF6 3L F63MF63NF63PF63QF63RF63SF63TF63VF63WF63YWT L9 2C L9 2D L9 2E L9 2F L9 2G L9 2H L9 2I L9 2K L9 2M L9 2N L9 2P L9 2Q L9 2R L9 2S L9 2T L9 2V L9 2W L9 2YWTF150CF150DF150EF150GF150HF150IF150KF15 0L F150MF150NF150PF150QF150RF150SF150TF150VF150WF150Y1201008060Relative ... of the ligands,all structures were converted from isomeric SMILES(Simpliﬁed Molecular Input Line Entry System) to 3Dstructures using CORINA [46]. Partial charges of theligands were calculated ... residues will enable manipu-lation of these SGLs by rational design or molecularevolution methods to obtain the desired catalyticactivity.Overexpression of heterologous proteins often resultsin...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: electron transfer through the nitric oxide synthase ﬂavoprotein domain pdf

... formodelling the dynamics, steady states and control of biochemical and other systems. Comput Appl Biosci 9,563–571.67 Noble MA, Munro AW, Rivers SL, Robledo L, DaffSN, Yellowlees LJ, Shimizu ... peptide, Garcin et al. [22] constructed a modelfor full-length nNOS that indicates that an allowablelarge motion of the FMN module could bring theFMN cofactor within an acceptable electron-transferdistance ... The autoinhibitory controlelement and calmodulin conspire to provide physiologi-cal modulation of endothelial and neuronal nitric oxidesynthase activity. Acta Physiol Scand 168, 53–63.30 Montgomery...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: probes of hydrogen tunnelling pptx

... many of these reactions will occur, in part, or in full, by quantum mechanicaltunnelling. We are particularly interested in the role of promoting vibra-tions on H transfer, and the Old Yellow ... environmental coupling ⁄ promotingvibrations in H-transfer reactions in the Old YellowEnzyme (OYE) family of ﬂavoproteins.Hydrogen tunnellingBecause of wave ⁄ particle duality, electrons and lightatoms ... dependence (B) of amodelled H-transfer reaction. The data are modelled using Eqn (10)with kH=5s)1, a KIE of 5 and various values of DDHà.S. Hay et al. Hydrogen tunnelling in biological systemsFEBS...

Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of ﬂavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc

... domain includes resi-dues 139–303 and consists of a core of ﬁve parallelb strands surrounded by seven a helices [62]. FAD isbound outside the antiparallel b barrel, and its isoal-loxazine lies ... of electrostatic repulsion in the control of Eox ⁄ sq, and particularly of Esq ⁄ hq, has also been demonstrated withseveral Flds [25,37,41,48–53]. Electron nuclear doubleresonance and 1D and 2D electron ... I92 replacedby A, E and K and D96N and also some multiplecharge reversal mutants), electron transfer from PSI toFldoxtakes place via a collisional-type mechanism[48,53,91]. Noticeably, some...

Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt

... for allosteric activation by GTP, although theoverall structure is highly similar to the other bacterialUMPKs. The relative simplicity of the apparent regula-tion of this structurally complex ... thank Andrea Hinas and FredrikSo¨derbom (Department of Molecular Biology, SwedishUniversity of Agricultural Science) for help with muta-genesis, Hans Eklund (Department of Molecular Biol-ogy, ... Department of Anatomy, Physiology and Biochemistry, Swedish University of Agricultural Sciences, Uppsala Biomedical Centre, Sweden2 Department of Molecular Biology, Swedish University of Agricultural...

Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx

... viral cap-sid jellyroll topology. J Mol Biol 228, 220–242.27 Anand K, Pal D & Hilgenfeld R (2002) An overview on2-methyl-2,4-pentanediol in crystallization and in crys-tals of biological ... jellyroll domain (Fig. 3). Antiparallelb-sheets made up of strands CHEF and BIDG packagainst each other, forming the jellyroll topology,a typical viral capsid protein fold [26]. The distaldomain ... of the protein solution at a concentra-tion of 5–10 mgÆmL)1was added to 1 lL of well solution.Small crystals grew within 2–3 months, reaching dimensions of 50 · 50 · 50 lm. Microseeding of...

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