... (2007) Accumulation of polyadenylated mRNA, Pab1p, eIF4E, and eIF4G with P-bodies in Saccharomyces cerevisiae. Mol Biol Cell 18, 2592–2602. 34 Hoyle NP, Castelli LM, Campbell SG, Holmes LEA & Ashe ... Stress-dependent relocalization of translationally primed mRNPs to cytoplasmic granules that are kinetically and spatially distinct from P-bodies. J Cell Biol 179, 65–74. 35 Buscagli...
Ngày tải lên: 15/03/2014, 23:20
... structural complexes of two N-acylsulfonamide-linked nucleosides with RNase A closely mimic the binding by nucleic acids. The attributes and versatility of N-acylsulfonamidyl and sulfonimidyl groups ... information The following supplementary material is available: Fig. S1. Atom numbering for compounds 6 and 7. Table S1. Torsion angles of nucleosides in RNase AÆ N-acylsulfonamidel...
Ngày tải lên: 14/02/2014, 22:20
Tài liệu Báo cáo khoa học: Active and regulatory sites of cytosolic 5¢-nucleotidase doc
... that is correctly folded and a large amount of incorrectly folded and insoluble protein. It is conceivable that a cN-II protein that is incorrectly folded but still soluble is also produced. A ... dell’Istruzione, dell’Universita ` e della Ricerca and by local funds from the University of Pisa. References 1 Tozzi MG, Camici M, Pesi R, Allegrini S, Sgarrella F & Ipata PL (1991) N...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Structural and functional studies of the human phosphoribosyltransferase domain containing protein 1 docx
... protein crystallography. Acta Crystallogr D Biol Crystallogr 50, 760–763. 31 Vagin A & Teplyakov A (2000) An approach to multi- copy search in molecular replacement. Acta Crystallogr D Biol Crystallogr ... coli BL21(DE3) strain and stored at )80 °C for further use. One hundred and fifty milliliters of LB supplemented with 50 lgÆmL )1 kanamycin was inoculated and grown at 37 °C over...
Ngày tải lên: 15/02/2014, 01:20
Tài liệu Báo cáo khoa học: Isolation and molecular characterization of a novel D-hydantoinase from Jannaschia sp. CCS1 docx
... whole-cell conversion of d ,l- p-HPH was also WT F63C F63D F63E F63G F63H F63I F63K F6 3L F63M F63N F63P F63Q F63R F63S F63T F63V F63W F63Y WT L9 2C L9 2D L9 2E L9 2F L9 2G L9 2H L9 2I L9 2K L9 2M L9 2N L9 2P L9 2Q L9 2R L9 2S L9 2T L9 2V L9 2W L9 2Y WT F150C F150D F150E F150G F150H F150I F150K F15 0L F150M F150N F150P F150Q F150R F150S F150T F150V F150W F150Y 120 100 80...
Ngày tải lên: 18/02/2014, 08:20
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: electron transfer through the nitric oxide synthase flavoprotein domain pdf
... for modelling the dynamics, steady states and control of biochemical and other systems. Comput Appl Biosci 9, 563–571. 67 Noble MA, Munro AW, Rivers SL, Robledo L, Daff SN, Yellowlees LJ, Shimizu ... peptide, Garcin et al. [22] constructed a model for full-length nNOS that indicates that an allowable large motion of the FMN module could bring the FMN cofactor within an acceptable ele...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: probes of hydrogen tunnelling pptx
... many of these reactions will occur, in part, or in full, by quantum mechanical tunnelling. We are particularly interested in the role of promoting vibra- tions on H transfer, and the Old Yellow ... environmental coupling ⁄ promoting vibrations in H-transfer reactions in the Old Yellow Enzyme (OYE) family of flavoproteins. Hydrogen tunnelling Because of wave ⁄ particle duality, electr...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Structural and mechanistic aspects of flavoproteins: photosynthetic electron transfer from photosystem I to NADP+ doc
... domain includes resi- dues 139–303 and consists of a core of five parallel b strands surrounded by seven a helices [62]. FAD is bound outside the antiparallel b barrel, and its isoal- loxazine lies ... of electrostatic repulsion in the control of E ox ⁄ sq , and particularly of E sq ⁄ hq , has also been demonstrated with several Flds [25,37,41,48–53]. Electron nuclear double re...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Structural and functional investigations of Ureaplasma parvum UMP kinase – a potential antibacterial drug target ppt
... for allosteric activation by GTP, although the overall structure is highly similar to the other bacterial UMPKs. The relative simplicity of the apparent regula- tion of this structurally complex ... thank Andrea Hinas and Fredrik So ¨ derbom (Department of Molecular Biology, Swedish University of Agricultural Science) for help with muta- genesis, Hans Eklund (Department of Molecul...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Structural and biochemical characterization of a human adenovirus 2/12 penton base chimera pptx
... viral cap- sid jellyroll topology. J Mol Biol 228, 220–242. 27 Anand K, Pal D & Hilgenfeld R (2002) An overview on 2-methyl-2,4-pentanediol in crystallization and in crys- tals of biological ... jellyroll domain (Fig. 3). Antiparallel b-sheets made up of strands CHEF and BIDG pack against each other, forming the jellyroll topology, a typical viral capsid protein fold [26]. The dis...
Ngày tải lên: 19/02/2014, 06:20