Báo cáo khoa học: "The role of photoperiod and temperature in the induction and the release of dormancy in Pinus sylvestris L seedlings" potx

... IDM (10 lgÆmL )1 insulin, 1 lm dexamethasone, and 0.5 mm IDM). Cells were then maintained in DMEM con- taining 10% fetal bovine serum and 1 lgÆmL )1 insulin. The medium was replaced every other ... biologically relevant role of miR-27 in the regulation of adipose tis- sue functions in vivo, we examined the expression of miR-27 in the genetically obese ob ⁄ ob mice....

Ngày tải lên: 18/02/2014, 08:20

... lgÆmL )1 of radioactively labelled 125 I-labelled MSSAE in binding buffer (DMEM containing 1 mgÆmL )1 BSA and 25 m M Hepes at pH 7.5). At increasing time intervals, cells were washed repeatedly with NaCl/P i andthentreatedwith0.6 M NaCl in ... by linking the exposed sulfhydryls to small thiols through formation of mixed disulfides. We found that sulfhydryls from cell monolayers an...

Ngày tải lên: 20/02/2014, 11:20

... results obtained by other groups who also point to the role of the connecting loop of the b-hairpin of defensins. A combination of mutational analysis [35] and structural analysis of the plant ... infer whether the key role of the b-hairpin loop of MGD1 (loop 3) in antimicrobial activity of MGD1 is of general value in the mechanism of action of de...

Ngày tải lên: 20/02/2014, 11:20

... [10]. Further interest in the aromatic stacking is in the role of the electrostatic properties in enzymatic catalysis of API, and in distinguishing the functionally catalytic quadruple Ser194–His57–Asp113– His210 ... remained parallel with the side-chain of His210. On the other hand, a small side-chain at position 169, typically W169V and W169A, deviates from...

Ngày tải lên: 21/02/2014, 03:20

... and specialization events through their evolution. In an attempt to relate MT functional performance at the molecular level (metal-binding abilities) and the role of MT at the physiological level (metabolic ... Thus, the overall available information suggests that: (a) C. elegans MTs are most likely involved in basic biological processes and (b) the role of CeMT1...

Ngày tải lên: 07/03/2014, 02:20

... for cholesterol in adrenal cells ([40,54]). In addition to normally cycling between the PM and other cellular organelles, free ergosta-5-eneol also must be avail- able in the yeast cell to serve ... results in an increased level of free sterol (Fig. 4B), and it would also be expected to increase the cycling of sterols by esterification and hydrolysis, resulting in an...

Ngày tải lên: 08/03/2014, 08:20

... reesei )in lanes 1 and 7, ScMaL pool I, containing the more heavily overgly- cosylated laccase, in lanes 2 and 5, and the less overglycosylated laccase ScMaL pool II in lanes 3 and 6. Function of ... the folding of the protein during the post-translational processing, and thereby the function of the mature laccase. Our structural analysis of wild-type MaL...

Ngày tải lên: 16/03/2014, 00:20

... formation of MRN foci [19–21]. The Mre11-binding domain of human Nbs1 has been localized to amino acids 682–693 in the COOH- terminal region of the protein. Deletion of this region of Nbs1 results in ... contains an intact Mre11-binding domain. Differences in the nature of the Nbs1 muta- tion, as well as in the dose of radiation administered to NBS cells, thu...

Ngày tải lên: 16/03/2014, 13:20

... hydrolysis and transport are proba- bly uncoupled by cleavage of the linker [22]. Although these results provided valuable suggestions for the role of the P-gp linker region, the molecular details, ... kinetic analy- ses of the native and linker-cleaved P-gp. In the present study, we investigated the functional role of the linker region of human P-gp using...

Ngày tải lên: 29/03/2014, 23:20

... (TPBS), and 100 lL of mAbs solutions in NaCl ⁄ P i were added to the wells and incubated for 60 min at 37 °C. After washing of the plate, 100 lL aliquots of a 1 : 1000 solution in TPBS of the rabbit ... & Medved L (2002) Interaction of fibrin(ogen) with the endothelial cell receptor VE-cadherin: mapping of the receptor-binding site in the NH2-terminal po...

Ngày tải lên: 30/03/2014, 03:20