... is, in principle, an auto-mated HPLC apparatus equipped with an auto-sampler, and can be purchased from a variety ofcompanies; and, at least in our hands, HPLC solventgradients and flow rates ... Protein-reactive natural products. Angew Chem Int Ed Engl 44,5788–5809.72 Zhang H, Yan W & Aebersold R (2004) Chemicalprobes and tandem mass spectrometry: a strategy for the quantitative analysis ... either incubated with recombinantHIV-1 protease or left untreated (control). Followingprotease incubation, both proteomes are S-alkylated and acetylated, and equal amounts are then mixed and subjected...