... isopropylamine, n-butylamine, n-amylamine, n-hexylamine, 1,6-diamino-hexane, and spermidine. Interestingly, the enzyme wasunable to use ammonia as a substrate and was distinctfrom alanine dehydrogenase ... Toyobo (Osaka, Japan), and New EnglandBiolabs (Beverly, MA, USA). All other reagents were of analytical grade from Nacalai Tesque (Kyoto, Japan) andWako Pure Chemical Industries (Osaka, Japan).Culture ... Cusa E, Obradors N, Baldoma L, Badia J & Aguilar J(1999) Genetic analysis of a chromosomal region con-taining genes required for assimilation of allantoinnitrogen and linked glyoxylate...