Báo cáo y học: "Expression analysis of three isoforms of hyaluronan synthase and hyaluronidase in the synovium of knees in osteoarthritis and rheumatoid arthritis by quantitative real-time reverse transcriptase polymerase chain reaction" potx

... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Figure 3). Flow cytometry analysis ... myriad of complications arising from the weakening of the effectiveness of the immune system with age [1-9]. One of the most prominent landmarks of the ag...

Ngày tải lên: 03/11/2012, 11:35

... substrate-accessing channel, and their guanidinium and hydroxyl groups, respectively, play crucial roles in the binding of the substrate by interacting with the carboxyl group of the substrate fatty acids ... for fixing the carboxylate of fatty acids is maintained by replacing Arg with Lys. However, hydrophobicity at the entrance to P450foxy should be significantly i...

Ngày tải lên: 08/03/2014, 22:20

... supernatant and inclusion body. Part of the production was applied to 12% SDS–PAGE analysis and Western blot analysis. (A) SDS-PAGE analysis: Most of the fusion protein was found in inclusion body. The ... treat type 2 daibetes by improving both insulin resistance and insulin secretion deficiency. However, we only ob- served the glucose-lowering effect of the...

Ngày tải lên: 25/10/2012, 11:10

... apoptosis by inhibiting the expression of Bcl-2 and by modulating phosphoryla- tion of JNK. In conclusion, various pro- and antiapop- tosis-related proteins were expressed in the process of hypoxia-induced ... identify key molecules in the course of hy- poxia-induced apoptotic pathways. Such analysis of trophoblastic cells will, in the near future, yield...

Ngày tải lên: 31/10/2012, 15:12

... chain reaction The common exons 2 to 11 and the three known variants of exon 1 of the hCDC4 gene (Figure 1A) were amplified by polymerase chain reaction (PCR). Primers were synthesized by ... Biochemistry of the Charité, Berlin, Germany using the ABI PRISM Big Dye Terminator system (Applied Biosystems, Germany). Sequences were analyzed using the Chromas s...

Ngày tải lên: 31/10/2012, 16:49

... omission of the primary or the secondary antibody or primary incubation in the presence of non-immunized rabbit serum instead of the primary antibody. Cell quantification and Statistical analysis ... hPMS2 and GTBP/hMSH6 are very important in distinguishing and repairing misparing and slippage errors in DNA synthesis. The mismatch is detected by two compl...

Ngày tải lên: 03/11/2012, 09:54

... found in DM43 first three domains and are present in the inhibitory receptors of Fig. 6. Inhibition of in vitro cytotoxicity of myotoxins I or II by DM64. Cytotoxicity was analyzed in vitro using ... sequences. The conserved aromatic residues phenylalanine and tyrosine typical of the Ig-fold are bold in each domain. Fig. 5. Inhibition of in vivo myotoxicity...

Ngày tải lên: 21/02/2014, 01:21

... protein as judged by SDS/ PAGE. The activity of the pure recombinant protein was 80% of that of t he wild-type protein in both the lysozyme and chitinase assays. Attempts to further purify the recombinant ... between the se two classes. Role of Tyr183 in catalysis In previous crystallographic studies it was shown that the hydroxyl side chain of Tyr183 is with...

Ngày tải lên: 22/02/2014, 04:20

... of the C-terminal extension has the potential to perturb function and reduce solubility of these proteins and their complexes with target proteins [15–19]. The N-terminus, which may be partly ... proposed for these proteins [7,8]. The a-crystallin domain, located toward the C-terminus of the protein monomer, consists of 80–100 amino-acid residues and is important for...

Ngày tải lên: 22/02/2014, 04:20

... in ammogenic activity of the intact molecule [6]. The presence of longer (compared to the highly endotoxic lipid of E. coli )chain fatty acids, the absence of phosphates and of an acyl group at O-3 of a-GlcN ... p.p.m.), while acyl 4 had no substituents. The signals of acyl chains could only be identified up to C-4, H-4, because of the overlap of the remaini...

Ngày tải lên: 08/03/2014, 09:20