... by cor-responding Sn,S2,S1,S1¢,S2¢,Sn¢ subsites of < /b> the < /b> enzyme. The < /b> scissilepeptide bond is located between the < /b> P1 and < /b> P1¢. Protease substrateresidues and < /b> subsites of < /b> the < /b> enzyme substrate-binding ... detected, probably as a result of< /b> the < /b> precipitation of < /b> the < /b> highly hydrophobic VVA productfollowed by its release from the < /b> parent VVASQLRANISHpeptide.To assess the < /b> thermal stability of < /b> C5a peptidase, ... 200 lM of < /b> the < /b> pNA peptidesubstrate was incubated in quartz cell for 60 min eitheralone or in the < /b> presence of < /b> 1 lM of < /b> C5a peptidase whilemonitoring hydrolysis by measurement of < /b> absorbance at405...