Báo cáo y học: "Microarray analysis of gene expression in lupus" ppt

... [18]. The microarray assay used (Panorama Cytokine Gene Array membranes; Sigma Genosys, Inc) included 375 genes enriched in cytokines, chemokines, cell surface 286 ably derive from studies of tissue samples ... among the IFN-induced genes overexpressed in the JDM muscle samples. Increased expression of several genes, including G1P3 (encoding IFN-induced 6-16) and CDKN1A (p21 cycli...

Ngày tải lên: 09/08/2014, 01:23

... against this pro-inflammatory cytokine. Interestingly in our PLCH patients this mechanism was down-regulated. The results of our proteome analysis of PLCH BAL suggested the involvement of some ... phosphorylase, pyruvate kinase isozymes, fibrinogen gamma chain, alpha 1B glycoprotein and actin cytoplasmic 1 were identified. BAL proteome analysis of PLCH patients also revealed...

Ngày tải lên: 10/08/2014, 09:22

... pronounced myelin degenera- tion as seen in the photochemically induced ischemic injury of sciatic nerve [33-35]. Increasing of nerve fiber myelin sheath thickness and especially CSA of axon may Table ... the analysis of hydroxyproline in biological tissues. Clin Biochem 1996, 29:225-229. 30. Edward CA, O'Brien WD: Modified assay for determination of hydroxyproline in...

Ngày tải lên: 10/08/2014, 10:20

... Department of Science and Technology of Zhejiang Province, China to Y. D. Yang, grant no. 2009C33009 from the Department of Science and Technology of Zhejiang Province, China to L. Zheng and grant no. Y2 00708441 ... provided analytical tools and were also involved in editing the manuscript, YYD designed the study and wrote the manuscript. Competing interests The authors declare t...

Ngày tải lên: 12/08/2014, 01:21

... may be differently regulated by inflammatory cytokines, suggesting a dynamic interplay that might have a potential role in airway inflammation. Background Allergic asthma is characterised by ... airway hyperreactivity in animal studies [12-14], they may have distinct roles in allergen-dependent airway obstruction [24]. Hence, NGF may play a role in the early airway response (EAR) in...

Ngày tải lên: 12/08/2014, 16:20

... cells in the blood, was shown to be signifi- cantly over-expressed in this study. An increase in PAI-1 suppresses the generation of plasmin resulting in hypofi- brinolysis and a relative hypercoagulable ... significantly by microarray analysis and RT-PCR results but immunohistochemical study failed to show an increased cell surface expression of these genes. Comparing the gen...

Ngày tải lên: 12/08/2014, 14:22

... immunoglobulin chains were also found to be significantly increased in aged thymocytes. Understanding the changes in gene expression in thymocytes with age may hold the key in determining thymocyte ... levels of many of these genes identified in the array analysis. Moreover, Western blot analysis of thymo- cyte lysates also confirmed that IgM increases with age (Fig...

Ngày tải lên: 03/11/2012, 11:35

... immediately upstream of the TATA box in the p-126 minimal promoter construct completely abolished the stimulation of luciferase activity by CDCA in HepG2 cells (Fig. 6). Interestingly CDCA reduced the activity of ... potentially be involved in estrogen-mediated modification of Bsep gene transcription. In analogy to the human choline acetyltransferase and the lipoprotein lipas...

Ngày tải lên: 17/03/2014, 23:20

... of the Sin3 binding domain of Mad in silencing gene expression when fused to DNA binding proteins has been demonstrated in two studies. One groupfusedtheMadSIDtothec-Jun DNA binding domain and ... gonadotropin responsiveness and inhibin expression [8]. In addition, KK1 cells are easy to maintain in culture and can be transfected with high efficiency . The data generated usin...

Ngày tải lên: 20/06/2014, 07:20

... member of DEAD/H box family, has conserved C-terminal helicase domain U68536 ZNF24 Zinc finger protein 4.0 1.4 2.8 Zinc finger protein 24; contains zinc fingers B. Genes overexpressed in healthy controls Gene ... involved in a given pathophysiol- ogy. Our analysis identified key regulators of innate immu- nity and inflammation including the proinflammatory mediators formyl peptide...

Ngày tải lên: 09/08/2014, 01:23