Báo cáo y học: "α α Upregulated hypoxia inducible factor-1α and -2α pathway in rheumatoid arthritis and osteoarthritis" pps

... that in OA ST. Nevertheless, pFAK staining in RA ST lining and sublining was significantly higher than in ND STs. We additionally showed Figure 4 Immunostaining of pPaxillin is increased in rheumatoid ... the colocalization and activation of FAK, Pyk2, Src and paxillin in RA and OA patient's ST lining and sublining may be important for integrin-mediated signaling. p...

Ngày tải lên: 09/08/2014, 10:21

... haematoxylin and eosin stained sections. Immunohistochemistry for HIF-1 α and HIF-2 α expression The HIF- 1α and HIF- 2α proteins were detected using ESEE 122 (IgG 1 monoclonal antibody [mAb]; ... significant. Results HIF- α expression in synovial lining cells HIF- 1α and HIF- 2α expression was both cytoplasmic and nuclear in arthritic synovial tissues (Fig. 1a,b). The...

Ngày tải lên: 09/08/2014, 01:21

... Introduction Rheumatoid arthritis (RA) is a chronic condition, which leads to varying degrees of functional impairment and disability. In early stages, symptoms reflecting the inflammatory ... and the intra-assay and inter-assay coefficient of variation is <5%. The assay is not influenced by rheuma- toid factors. The serum concentrations of COMP obtained by this assay are highl...

Ngày tải lên: 09/08/2014, 01:21

... article Four-year follow-up of infliximab therapy in rheumatoid arthritis patients with long-standing refractory disease: attrition and long-term evolution of disease activity Bert Vander Cruyssen 1 , ... of increasing the dos- age by 100 mg [11,12]. The standard infliximab dosage of 3 mg/kg every 8 weeks was reinstituted in a majority of patients beginning in June 2002, the tim...

Ngày tải lên: 09/08/2014, 08:22

... E-cadherin in syn- ovial tissue [29], our study is in line with a study by Trollmo and colleagues [30], describing E-cadherin expression in the lining layer of healthy synovial tissue, indicating ... subjects. * indi- cates P < 0.05. Figure 4 α- Smooth muscle actin (α- sma) staining in healthy and arthritic synovial tissueα-Smooth muscle actin (α- sma) staining in healthy a...

Ngày tải lên: 09/08/2014, 08:23

... recombinant F-actin capping protein α- 1 subunit (CapZα-1) with His-tag and citrullination of the recombinant CapZα-1Preparation of recombinant F-actin capping protein α- 1 subunit (CapZα-1) with ... Biotechnol- ogy). F-actin capping protein α- 1 subunit (CapZα-1) was detected by Western blotting using an anti-CapZα-1 polyclo- nal antibody (Chemicon International, Temecula, CA, USA) an...

Ngày tải lên: 09/08/2014, 08:23

... target fused to a cytokine, thus retaining the functions of both the antibody and the cytokine. In cancer, the use of single-chain antibody fragments for targeting and in vivo imaging of tumors is ... neutralizing anti-TNF-antibodies in the treatment of rheumatoid arthritis has paved the way for using immunocytokines in non-lethal diseases. The study of Kathrin Schwager and...

Ngày tải lên: 09/08/2014, 14:22

... PLA 2 s in the PGE 2 -biosy- nthetic pathway, are also expressed in synovial lining cells in the RA joints. Distribution of sPLA 2 -V in the synovial sublining lesions (Fig. 5) is noteworthy because ... staining of sPLA 2 -IIE was observed in two inactive RA sections (Fig. 4A), it was intense in VSMC in three distinct active RA sections (Fig. 4Ba– c). In contrast, staining of...

Ngày tải lên: 19/02/2014, 16:20

... [6], prothymosin a [7], a bacterial ®bronectin-binding protein [8], the brain protein a-synuclein together with the related proteins b-synuclein and c-synuclein [9±12], a nd the brain protein tau ... e by a lkaline urea P AGE, with only the b-casein showing slight contamination with a glycosylated form of j-casein. Recombinant wild-type human a-, b-andc-synuclein, as well as the A30P and...

Ngày tải lên: 21/02/2014, 15:20

... activity and high activity did not show any difference. We could not find any correlation with sex and age (whole group/division into two patient groups ≤ 65 years and ≥ 66 years, P = 0.32), whereby ... tissue in rheumatoid joints and in normal syno- vial tissue. To our knowledge, no study has previously investigated the serum levels of cathepsin K in RA. Our results demonstra...

Ngày tải lên: 09/08/2014, 06:22