... Tyr. These mutations may in uence the shape and volume of the acyl binding pocketand thereby alter the binding mode and affinity of theenzyme for PAA and derivatives thereof, while maintainingthe ... phenylglycyl- and p-hydroxyphe-nylglycyl-enzyme of bF24A, as indicated by, respectively, atwofold and fourfold increased Vs/Vhratiocomparedtothewild-type (Table 4). The Vs/Vhratio using ... penicillin acylase; PAA, phenylacetic acid; PAAM,phenylacetamide; PAAOM, phenylacetic acid methyl ester; PG, phe-nylglycine; (H)PGA, (p-hydroxy)-D-phenylglycine amide; (H)PGM,(p-hydroxy)-D-phenylglycine...