Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf
Báo cáo y học: "Abnormalities of B cell phenotype, immunoglobulin gene expression and the emergence of autoimmunity in Sjögren’s syndrome" pdf
... in partby chemokines and the local expression of < /b> their receptors.Chemokines and the expression of < /b> chemokine receptorsby the inflamed tissue as well as by lymphocytes are there-fore likely ... concentration and maintenance of < /b> B cell activation in the salivary glands of < /b> patients with pSS leads to a significant depletion of< /b> memory B cells in the peripheral blood, probably resulting in autoantibody ... lymphocytes, Sjögren’s syndromeReviewAbnormalities of < /b> B cell phenotype, immunoglobulin gene expression and the emergence of < /b> autoimmunity in Sjögren’s syndromeThomas Dörner1 and Peter E Lipsky21Department...
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Báo cáo y học: "Cartilage oligomeric matrix protein deficiency promotes early onset and the chronic development of collagen-induced arthritis" pdf
... they have no competing interests.Authors' contributionsHG was responsible for the majority of < /b> the practical work and the writing of < /b> the manuscript. The study was originallydesigned by ... levels of < /b> antibodies against cartilage oligomeric matrix protein and collagen IIAntibody levels against COMP in serum were analyzed byenzyme-linked immunosorbent assay (ELISA) using recom-binant ... extracellular matrix glyc-oprotein and a member of < /b> the thrombospondin (TSP) family[8]. To date, five members of < /b> the TSP family have been identi-fied. Among them, TSP-1 and TSP-2 contain three...
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Báo cáo khoa học: "Parsing with Treebank Grammars: Empirical Bounds, Theoretical Models, and the Structure of the Penn Treebank" ppt
... beparsed, is the number of < /b> nonterminal categories in the grammar and is the number of < /b> (active) states in the FSA encoding of < /b> the grammar. The time boundis derived from counting the number of < /b> ... edges, of < /b> which there can be , one for ev-ery category and span, are a shrinking minority. Thisis because, while is bounded above by 27 in the tree-bank7(for spans 2), numbers in the thousands ... be many alignments whichdiffer only in the spans of < /b> the categories, but line up the same tags with the same words. However, there will bea certain number of < /b> unique ways in which the wordsand...
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Báo cáo khoa học: Ca2+-binding allergens from olive pollen exhibit biochemical and immunological activity when expressed in stable transgenic Arabidopsis pdf
... absence of < /b> this residue wasconfirmed by mass spectrometry after in- gel digestion of < /b> the protein with trypsin. The resulting peptides wereanalysed by MALDI-TOF. The molecular mass of < /b> one of < /b> these ... promoter in pROK2 plasmids followed by the nopaline synthase terminator(NT). These plasmids contain the NPTII gene between the nopalinesynthase promoter (NP) and the NT, as well as the left (LB) and right ... inhibitors for the polyclonalinhibition and from 10 lgÆmL)1 of < /b> inhibitors for the serainhibition were used. The binding of < /b> human IgE and IgGpolyclonal antiserum was detected as indicated above. Per-oxidase...
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Báo cáo hóa học: " Herpes Simplex Virus Type 1 Us3 Gene Deletion Influences Toll-like Receptor Responses in Cultured Monocytic Cells" pdf
... Roizman B: Herpes simplex virus 1 gene expression is accelerated by inhibitors of < /b> histone deacety-lases in rabbit skin cells infected with a mutant carrying acDNA copy of < /b> the infected -cell protein ... I B kinases IKKε and TANK-binding kinase 1(TBK1) leads to phosphorylation of < /b> IRF-3 by the kinases.IRF-3 translocates to the nucleus and induces severalgenes such as the IFN-β gene [2]. In ... purposes)Determination of < /b> the intracellular MxA proteinFor MxA intracellular staining, 1 × 106 of < /b> U937 cells wereinfected as described above. The U937 cells were stainedas described earlier [46]. Briefly,...
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Báo cáo Y học: RING finger, B-box, and coiled-coil (RBCC) protein expression in branchial epithelial cells of Japanese eel, Anguilla japonica pot
... [39]. The finding may open a newavenue leading to better understanding of < /b> the mode of< /b> action of < /b> not only eRBCC but also other members of < /b> the RBCC family through identification of < /b> their cellularsubstrates.Fig. ... sequence of < /b> the eRBCC RING finger domain and B- box domain with several mem-bers of < /b> the RBCC family is shown. The conserved Cys and His residuesare shown with asterisks. The zinc-coordinating Cys and ... specificity of < /b> eRBCC,we next prepared a number of < /b> recombinant E2 enzymes and examined their interaction with eRBCC. The ubiquitinationreaction was observed only in the case of < /b> UbcH5C,demonstrating...
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Báo cáo Y học: Conditionally immortalized adrenocortical cell lines at undifferentiated states exhibit inducible expression of glucocorticoid-synthesizing genes pot
... for syntheses of < /b> both aldosterone and corticosterone, were inducible in the three cell lines in tem-perature- and/ or dibutyryl cAMP-dependent manners.Notably, these cell lines displayed distinct ... P450c21 genes indicatethat the cell lines have the property of < /b> adrenocortical cells.Especially, expression of < /b> the e ndogenous P450c21 gene in the established cell lines contrasts strikingly with the characteristics ... undiffer-entiated cell layer.Although a number of < /b> cell lines were obtained in the present study, there was no cell line expressing the P450aldo gene. During initial passages, however, we found that the cells...
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Báo cáo y học: "Early rheumatoid arthritis is characterized by a distinct and transient synovial fluid cytokine profile of T cell and stromal cell origin" pptx
... withphosphate-buffered saline/0.05% Tween 20 and incubatedwith 25 µl of < /b> biotinylated detection antibody, diluted in 25 µlblocking buffer and 50 µl assay buffer (1% bovine serum albu-min [Sigma] in phosphate-buffered ... T cell derivedcytokines strongly suggests T cell activity in early RA. The clear difference between the levels of < /b> specific T cell relatedcytokines in early RA and other early inflammatory arthritides,as ... involved in drafting the manuscript. SJCacquired the cytokine and chemokine data, analyzed and inter-preted the data, and was involved in drafting the manuscript.ET acquired the cytokine and...
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