Báo cáo khoa học: " Rôle d’Hylobius abietis (L) (Col, Curculionidae) dans le transport de Leptographium procerum (Kendr) Wingf et son inoculation au pin sylvestre" pps

... larve d’H abietis Article original R le d’Hylobius abietis (L) (Col, Curculionidae) dans le transport de Leptographium procerum (Kendr) Wingf et son inoculation au pin sylvestre D ... suite de morsures de maturation de l’insecte. Des inoculations de L procerum au niveau de larges blessures, pratiquées artificiellement...

Ngày tải lên: 08/08/2014, 23:22

... d’après l’ampleur du houppier et la densité de son feuillage, d’après le degré de fermeture du peuplement au voisinage de l’arbre et d’après la densité et la hauteur de la ... dans la région composée des massifs siliceux des Maures et de l’Esterel (DRAF PACA, 1988). Le liège a fait, au siècle dernier et au début de ce siècl...

Ngày tải lên: 08/08/2014, 23:22

... le prétraitement. Les échantillons de la rondelle G, une fois décou- pés, ont été conservés 8 mois dans de l’eau + formol et les rondelles F et H conservées 10 j au congélateur ... découpe. Dans tous les autres cas le matériau, avant et après découpe, était conservé dans de l’eau changée régulière- ment. Tout cela est récapitulé da...

Ngày tải lên: 08/08/2014, 23:22

... antibodies against KAVKKDEL, KSEKKDEL or KXXXXXKDEL, which recognize the ER retention signal KDEL and bind to various ER-resident proteins, were obtained from S. Fuller (EMBL, Heidelberg, Germany). ... fractions was determined by the method of Lowry et al. [45]. Galactosyltransferase was assayed as described by Beaufay et al. [46]. Acid phospha- tase was assayed as described by Trouet [47...

Ngày tải lên: 19/02/2014, 02:20

... was measured as 7-ethoxyresourfin O-deethylase, methoxyre- sourfin O-demethylase and pentoxyresourfin O-dealkylase activities, respectively, using the method previously described by Burke et al. [42]. ... was detected using an ECL detection system (iNtRON Biotechnology) in accor- dance with the manufacturer’s instructions. The intensity of the immunoreactive bands was determined using densito-...

Ngày tải lên: 14/02/2014, 18:20

... developing disease, including gastric cancer, as summarized in excellent review articles [1–3,5] (more details are also provided in Doc. S1). During the last two decades, the cellular and molecular mechanisms ... severe malignant lesions. Therefore, it is clear that CagA and the T4SS play a central role in the pathogenesis of H. pylori in vivo. H. pylori in vitro infection models: T4SS-depend...

Ngày tải lên: 14/02/2014, 19:20

... entry into corneal fibroblasts (CFs) leads to changes in actin cytoskeleton [29]. We also decided to examine whether cytoskeletal changes played any significant role in HSV-1 entry into RPE cells. ... & Straus SE (2003) Roles for endocytosis and low pH in herpes simplex virus entry into HeLa and Chinese hamster ovary cells. J Virol 77, 5324–5332. 31 de Duve C, de Barsy T, Poole B, Troue...

Ngày tải lên: 18/02/2014, 14:20

... important role in skin wound healing. After a skin injury, PPARb expression is rap- idly elevated in the epidermis at the wound edges; dele- tion of a single PPARb allele results in delayed wound healing ... for 60 s; 32 cycles were used, depending on the abundance of DNA. Electrophoretic mobility shift assays PPRE studies were performed using biotin-labeled oligonu- cleotides and nuclear ext...

Ngày tải lên: 18/02/2014, 18:20

... 5¢-AGTTGAGGGGACTTTCCCAGGC-3¢) consensus double-stranded oligonucleotides were purchased from Promega. These oligonucleotides were labeled at the 5¢-end with 32 P by the T4 DNA polynucleotide kinase pro- vided in the labeling ... the 32 P-labeled oligonucleotide. For competition experiments, 3.5 pmol of cold oligonucleotide was added at the same time. After binding, protein–DNA complexes were...

Ngày tải lên: 19/02/2014, 06:20

... side chains, R397 and R120, are also involved in fosfomycin binding, and in view of the multitude of interactions, it was assumed that deletion of a single interaction would not lead to a complete ... N-(phosphonomethyl)-glycine; ITC, isothermal titration calorimetry; MurA, UDP-N-acetylglucosamine enolpyruvyl trans- ferase; TPCK, L -(tosylamido-2-phenyl) ethyl chloromethyl ketone; UDPNAG, UDP...

Ngày tải lên: 19/02/2014, 13:20