... were lysed with hypotonic lysis buffer with proteaseinhibitors (Roche/Boehringer Mannheim, Indianapolis,USA). Thirty micrograms of cytoplasmic lysates or 20 µgnuclear lysates were loaded onto ... influ-enced by cytokines. The proinflammatory cytokines tumornecrosis factor alpha, IL-1, and IL-6 augment osteoclasto-genesis [9–11], while IL-10, IL-12, IL-18, and IFN-γ anta-gonize osteoclastogenesis ... viareceptor activator of nuclear factor kappaB ligand-dependentand -independent mechanisms. J Bone Miner Res 2001, 16:328-337.46. Kobayashi N, Kadono Y, Naito A, Matsumoto K, Yamamoto T,Tanaka...