... cross-sectional technique [38]. Alizarin red-S andalcian blue staining were used to examine skeletalmalformations [9].ResultsAn increase in mortality was seen in the study on dose-incidence response, ... effects of ionizing radiation.United Nation, New York, 1977.38. Wilson,J.G.Embryological considerations in teratology. In: Teratology: principles and techniques, pp. 251- 261,(Wilson JG and ... mal-formations under dissection microscope. Fetuses were fixed in Bouin's solution, then stored in 70% ethanol. Thepresence of visceral malformations was determined usingWilson's cross-sectional...