... AccessCostunolide causes mitotic arrest and enhances radiosensitivity in human hepatocellular carcinoma cellsChia-Yuan Liu1,3, Hsun-Shuo Chang5, Ih-Sheng Chen5, Chih-Jen Chen3, Ming-Ling Hsu2, ... nucleus in a round cell contour. These findings indicated a mitotic arrest at the metaphase was induced by costunolide.Signaling molecules associated with mitosis arrest As demonstrated in Figure ... cytometer, mitotic index by Liu’s stain and morphological observation, mitotic spindle alignment by immunofluorescence of alpha-tubulin, expression of cellcycle-related proteins by Western blotting, and...