... Â-diacyl-1,3-diaminopropyl-2-carbamoyl bivalent cationic lipids for gene delivery synthesis, in vitro transfection activity, and physicochemical characterization Michael Spelios and Michalakis SavvaDivision ... 0001 2001 4001 0001 2001 400+ /– 1:1 + /– 2:1 + /– 4:1 liposomes + /– 1:1 + /– 2:1 + /– 4:1 liposomes+ /– 1:1 + /– 2:1 + /– 4:1 liposomes + /– 1:1 + /– 2:1 + /– 4:1 liposomesD (nm)D (nm)1,3lb11,3lb21,3lb31,3lb41,3lb502004006008001,3lb11,3lb21,3lb31,3lb41,3lb5 –8 0 –6 0 –4 0 –2 0 –8 0 –6 0 –4 0 –2 0020406080Zeta ... PL(1994) Enhanced gene delivery and mechanism studieswith a novel series of cationic lipid formulations. J BiolChem 269, 255 0–2 561.M. Spelios and M. Savva Novel cytofectins for gene delivery FEBS...