Báo cáo y học: " BioShuttle-mediated Plasmid Transfer" pot

... would have very profound implications for human germline gene therapy. Gene transfer into embryos (using pronuclear microinjection or RVVs) is inherently very costly and technically demanding, ... Similarly, the pronuclei in ovine zygotes are very difficult to visualise, due to sharing a very similar refractive index with the cytoplasm; this necessitates the use of top-quality optics, ......

Ngày tải lên: 03/11/2012, 10:01

... from R&D Systems (Minneapolis, MN) and displayed two bands at 18 kD and 23 kD. This difference may be due to dissimilar cell types, which may produce different numbers of glycosylation sites. ... may be expressed by their adenoviral vectors in AN and SD rats, the osteogenic potentials of BMP4 or BMP6 are significantly reduced in immunocompetent rats. This limitation may be caused b...

Ngày tải lên: 31/10/2012, 17:08

... 5200 (RLYR) 2 1950 (RLYR) 4 338 (RLYR) 8 48 Dendritic R4 (RLYR) 2 K 3700 (RLYR) 4 K 2 K 1510 (RLYR) 8 (K 2 K) 2 K 1514 Dendritic R8 RLYRKVYG 3950 (RLYRKVYG) 2 K 1420 (RLYRKVYG) 4 K 2 K 610 (RLYRKVYG) 8 (K 2 K) 2 K 112 Tachyplesin 108 928 ... determined accurately because o f its poor solubility in phosphate buffers, this peptide rapidly and quantitatively induced erythrocytes aggregations. Pr...

Ngày tải lên: 22/02/2014, 04:20

... SalIsitesofthe pQE-32 plasmid (Qiagen, GmbH, Germany) resulting in the generation of the pQE-32-Mydj2 plasmid. The accuracy of the resu lting construct ( pQE-32-Mydj2) was v erified by DNA sequencing, and the plasmid ... subsequently used to overexpress M ydj2–6·His in Escherichia coli. Expression and purification of histidine-tagged Mydj2: antibody production The p QE-32-Mydj2 plasmid w...

Ngày tải lên: 17/03/2014, 17:20

... CO 2 Acetaladehyde fi acetate Glyceraldehyde 3-P fi glycerol Formation of Acetyl-CoA in the cytosol Acetate fi acetyl-CoA (cytosolic) Anaplerotic reaction (cytosolic) Pyruvate (cytosolic) + CO 2 fi ... 6-P EMP-pathway Glucose 6-P fi fructose 6-P (reversible) Fructose 6-P fi 2 glyceraldehyde 3-P Glyceraldehyde 3-P fi phosphoenolpyruvate Phosphoenolpyruvate fi pyruvate (cytosolic) PP-pathway Glucose .....

Ngày tải lên: 31/03/2014, 23:20

... MMP-2, the clinical phenotype is not yet explained. We still do not know precisely what role MMP-2 plays in human biological processes. In vitro MMP-2 can cleave type I and type IV collagens, but ... MMP- 13 lacking this catalytic domain has no enzymatic activity. The MMP-13 –/– mice are fertile, and they grow and survive normally. The major developmental phenotype includes widened growth ......

Ngày tải lên: 09/08/2014, 01:21

... then mechanically dis- solved by vortex-mixing and further analysed for DNA, gly- cosaminoglycan and hydroxyproline content as described previously [15]. All biochemical analyses was performed ... stained positive at days 7 and 14. The other samples were only weakly positive at day 14. To test the potential of using Hyaff-11 as a scaffold for the delivery of chondrocytes, the scaffold was ......

Ngày tải lên: 09/08/2014, 06:22

... at least in mice, early IL-4 production by naïve Th cells can also be induced by IL-25, a cytokine that is produced not only by Th2 cells but also by an unidentified cell type found in the gut ... encephalomyelitis (EAE). J Immunol 1996, 156:5- 7. 46. Kageyama Y, Koide Y, Yoshida A, Uchijima M, Arai T, Miyamoto S, Ozeki T, Hiyoshi M, Kushida K, Inoue T: Reduced susceptibility to collagen-in...

Ngày tải lên: 09/08/2014, 10:23

... Medicine, Yamaguchi University, Ube, Yamaguchi, 755-8611, Japan 4 Department of Orthopaedic Surgery, Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan Corresponding author: Yasuaki ... Graduate School of Medicine, Kyoto University, Kyoto, 606-8507, Japan 2 Department of Orthopaedic Surgery, National Hospital Organization Kyoto Medical Center, Kyoto, 612-8555, Japan 3 Depar...

Ngày tải lên: 09/08/2014, 14:21

... cell apoptosis in the rheumatoid synovium [6]. Synovial mast cells produce tissue destructive proteases and pro-inflammatory cytokines, most prominently TNF-α, and they therefore represent an interesting ... however, that the efficacy analysis of this phase II study is hampered by the lack of a placebo group. Thus, a significant proportion of the study population could have improved by regres...

Ngày tải lên: 09/08/2014, 14:21