... (PUFA) (AA, EPA or DHA) for 24 h and then incubated with interleukin-b (IL-1b)(10 ngÆmL )1 ,24 h)withor withou t the mitogen-activated protein kinase kinase (MEK) i nhibitor, U0 126 (U, 50 lM ), ... iCycler(Bio-Rad), according to the manufacturer’s instructions, and cycle parameters were: 50 °C(2min )and9 5°C(10 min), followed by 40 c ycles of 95 °C(15s),60°C(30 s ) and 72 °C (30 s). V ariations ... (ERK1 / 2) in AA-induced proliferation using cells incubated w ith t heMAPK kinase (MEK) inhibitor, U0 126 . U0 126 completelyinhibited the cyclin D1 promoter activity in AA-pretreatedSMC incubated...