... oxyhemeappeared at 540 and 579 nm. Then, a broad bandappeared at around 660 nm, and was maximal 9–12 minafter initiation of the reaction. The spectral features of the final reaction mixture were analogous, ... vanishedafter 30 min, and instead, an absorption peakappeared at 637 nm, suggesting the formation of CO–verdoheme. The 637 nm band disappeared gradu-ally and was replaced by a new broad band ... T, Zhang X, Sun D,Sato M, Sasahara M, Kayama T, Ikeda-Saito M &Yoshida T (2000) Histidine 20, the crucial proximalaxial heme ligand of bacterial heme oxygenase Hmu Ofrom Corynebacterium...