Báo cáo khoa học: "The relative importance of cork harvesting and climate for stem radial growth of Quercus suber " doc

... G. Oliveira et al .Stem growth of Quercus suber L. Original article The relative importance of cork harvesting and climate for stem radial growth of Quercus suber L. Graça Oliveira * , ... slow growth of Q. suber, its large hetero - geneity (size, form, age) and the recurrent cork harvesting. Here we present a first study of the effects of c...

Ngày tải lên: 08/08/2014, 14:21

... with AxCALSKD EQ at an MOI of 0, 1000, or 2000, and AxCAN- Cre at an MOI of 150. After 24 h of incubation, the cells were washed twice with NaCl ⁄ P i and fixed with 4% parafor- maldehyde in NaCl ⁄ P i for 15 min. ... total amount of protein in cells decreased to 87% after 48 h of incubation, the amount of glycoprotein decreased more. The results of culturing for 48 h sho...

Ngày tải lên: 30/03/2014, 04:20

... and GST- lofMPK6. However, when lofMPK3 and lofMPK6 proteins were tested for phosphorylation with OXI1, no phosphorylation of lofMPK3 or lofMPK6 was observed (Fig. 5A). On the other hand, when ... 1% sucrose and 0.7% agar. The seeds were stratified at 4 °C for 72 h and were then transferred to 22 °C under long day conditions (16 h light, 8 h dark) for germination and growth....

Ngày tải lên: 14/02/2014, 19:20

... isolated isoforms of the bean PAL after chromatofocusing are enzymes with increasing number of Tyr-loop-out conformations at the four active sites. The isoelectric points of these forms can be ... of inactivation of PAL by enfor- cing the Tyr-loop-out conformation which is catalyti- cally inactive and more sensitive to degradation. The presence of conformationally stable Tyr-loo...

Ngày tải lên: 07/03/2014, 12:20

... cycle of 95 °C for 2 min (Hot- Start), followed by 10 cycles of 95 °C for 30 s, 58.7 °C for 30 s and 72 °C for 2 min, followed by 25 cycles of 95 °C for 30 s, 58.7 °C for 30 s and 72 °C for 2 ... loss of CUX1-formed complexes, as observed for sites 8, 10, 12, 13, 14 and 15 and, to a lesser extent, for site 3 (Fig. 2B). The addition of an excess amount of n...

Ngày tải lên: 29/03/2014, 21:20

... conception and design of the study and substantially contributed to interpretation of data, drafting of the manuscript, critical revision of the manuscript and final approval. All authors read and approved ... synthesized and provided ErPC and ErPC3 for the analysis. WB significantly contributed to data analysis. CB participated in the conception of the study and int...

Ngày tải lên: 09/08/2014, 10:20

... to thank Kevin Howland and Judy Hardy (Department of Biosciences, University of Kent, Can- terbury, UK) for synthesis of the peptides, and the Wellcome Trust for establishment of a Protein Science Facility. ... per- formed as described for SurA [11]. Cloning and protein expression of human PDI were performed as described previ- ously [11]. [ 125 I]-Bolton–Hunter labellin...

Ngày tải lên: 16/03/2014, 06:20

... frequency-inverse document fre- quency (Salton and Buckley, 1988), and log en- tropy (Landauer and Dumais, 1997). 3.4 Measurements The choice of similarity function for the vector space is the least standardized ... of models approximate 31 Lin Dekang. 1998. Automatic retrieval and clustering of similar words. In Proceedings of the Joint An- nual Meeting of the Association...

Ngày tải lên: 17/03/2014, 00:20

... (see Materials and methods) for 90 min, and then incubated with and without NaF for an additional 30 min at 30 °C before being tested for interaction with 14-3-3. Lanes 1 and 2, 1% of the lysate used for ... implications for the respective roles of centaurin-a and the 14-3-3 isoforms in the regulation of signalling through CKI isoforms. Materials and methods Materi...

Ngày tải lên: 23/03/2014, 04:20

... for prmt1 morphants. (A) Dorsal view of embryos (10-somite stage) for krox20 staining. The positions of r3 and r5 are indicated. The widths of r3 and r5 and the vertical distance between r3 and ... to form the precursors of the meso- derm and endoderm, and C ⁄ E, in which cells accumu- late on the dorsal side and lead to axis formation. Gastrulation begins at 50% of...

Ngày tải lên: 28/03/2014, 23:20