Báo cáo khoa học: "The relative importance of cork harvesting and climate for stem radial growth of Quercus suber " doc

... G. Oliveira et al .Stem growth of Quercus suber L.Original articleThe relative importance of cork harvesting and climate for stem radial growth of Quercus suber L.Graça Oliveira*, ... slow growth of Q. suber, its large hetero-geneity (size, form, age) and the recurrent cork harvesting. Here we present a first study of the effects of cork harvesting and climate on stem radial ... correlate Stem growth of Quercus suber L. 441Table I. Characteristics of the selected cork- oaks in each stand. Values at the beginning of the study (1989 at CJM; 1992 at HP).Stand Last cork harvesting...

Báo cáo khoa học: The relative contribution of mannose salvage pathways to glycosylation in PMI-deﬁcient mouse embryonic ﬁbroblast cells pdf

... withAxCALSKDEQat an MOI of 0, 1000, or 2000, and AxCAN-Cre at an MOI of 150. After 24 h of incubation, the cellswere washed twice with NaCl ⁄ Pi and ﬁxed with 4% parafor-maldehyde in NaCl ⁄ Pi for 15 min. ... total amount of protein in cells decreased to 87%after 48 h of incubation, the amount of glycoproteindecreased more. The results of culturing for 48 hshowed that the percentage of glycosylated ... were a linear gradient of 70% solvent A and 30% solvent B to 40% solvent A and 60% solvent B over20 min at a ﬂow rate of 0.8 mLÆmin)1, followed by 5 min of 40% solvent A and 60% solvent B to...

Tài liệu Báo cáo khoa học: The Arabidopsis protein kinase Pto-interacting 1-4 is a common target of the oxidative signal-inducible 1 and mitogen-activated protein kinases docx

... and GST-lofMPK6. However, when lofMPK3 and lofMPK6proteins were tested for phosphorylation with OXI1,no phosphorylation of lofMPK3 or lofMPK6 wasobserved (Fig. 5A). On the other hand, when ... 1% sucrose and 0.7% agar.The seeds were stratiﬁed at 4 °C for 72 h and were thentransferred to 22 °C under long day conditions (16 h light,8 h dark) for germination and growth. For stress ... of OXI1K45R and PTI1-4 by MPK3 as well as MPK6 proteins could bedetected (Fig. 5B). As expected, no phosphorylationwas seen when the kinase inactive forms lofMPK3 and lofMPK6 were tested for...

Báo cáo khoa học: The essential tyrosine-containing loop conformation and the role of the C-terminal multi-helix region in eukaryotic phenylalanine ammonia-lyases docx

... isolated isoforms of the bean PALafter chromatofocusing are enzymes with increasingnumber of Tyr-loop-out conformations at the fouractive sites.The isoelectric points of these forms can be ... of inactivation of PAL by enfor-cing the Tyr-loop-out conformation which is catalyti-cally inactive and more sensitive to degradation. Thepresence of conformationally stable Tyr-loop-outforms ... than aver-age (22 and 25 A˚2 for HAL and PAL, respectively).Because there is no indication for a Tyr53-loop-outstructure for HAL but between HAL and PAL struc-tural and mechanistic similarity...

Báo cáo khoa học: The Promyelocytic Leukemia Zinc Finger (PLZF ) gene is a novel transcriptional target of the CCAAT-DisplacementProtein (CUX1) repressor ppt

... cycle of 95 °C for 2 min (Hot-Start), followed by 10 cycles of 95 °C for 30 s, 58.7 °C for 30 s and 72 °C for 2 min, followed by 25 cycles of 95 °C for 30 s, 58.7 °C for 30 s and 72 °C for 2 ... loss of CUX1-formed complexes, as observed for sites 8, 10, 12, 13, 14 and 15 and, to a lesser extent, for site 3 (Fig. 2B). The addition of an excess amount of nonlabelled oligonucleotide for ... NP-40 and 0.5%Na-deoxycolate; 40 lg of total protein extract were ana-lyzed by 3–8% Tris acetate NuPAGE (Invitrogen) for thedetection of CUX1 and by 4–12% Bis-Tris NuPAGE for the detection of...

Báo cáo khoa học: " The membrane targeted apoptosis modulators erucylphosphocholine and erucylphosphohomocholine increase the radiation response of human glioblastoma cell lines in vitro" pdf

... conception and design of the study and substantially contributed tointerpretation of data, drafting of the manuscript, criticalrevision of the manuscript and final approval. All authorsread and approved ... synthesized and provided ErPC and ErPC3 for the analysis. WB significantly contributed todata analysis. CB participated in the conception of thestudy and interpretation of data. VJ performed conception and ... http://www.ro-journal.com/content/1/1/6Page 7 of 17(page number not for citation purposes)ErPC and ErPC3 reduce colony formation ability of T98G cells and increase radiation-induced eradication of clonogenic T98G...

Báo cáo khoa học: The periplasmic peptidyl prolylcis–transisomerases PpiD and SurA have partially overlapping substrate specificities doc

... to thank Kevin Howland and Judy Hardy(Department of Biosciences, University of Kent, Can-terbury, UK) for synthesis of the peptides, and theWellcome Trust for establishment of a Protein ScienceFacility. ... per-formed as described for SurA [11]. Cloning and proteinexpression of human PDI were performed as described previ-ously [11]. [125I]-Bolton–Hunter labelling of peptides was per-formed ... between 10% and 30% of control); +, weak competition (intensity of crosslinking productbetween 50% and 80% of control); ), no competition (intensity of crosslinking product > 80% of control).Peptide...

Báo cáo khoa học: "The S-Space Package: An Open Source Package for Word Space Models" pdf

... frequency-inverse document fre-quency (Salton and Buckley, 1988), and log en-tropy (Landauer and Dumais, 1997).3.4 MeasurementsThe choice of similarity function for the vectorspace is the least standardized ... of models approximate31Lin Dekang. 1998. Automatic retrieval and clustering of similar words. In Proceedings of the Joint An-nual Meeting of the Association for ComputationalLinguistics and ... J. Kristoferson, and A. Holst. 2000. Ran-dom indexing of text samples for latent semanticanalysis. In L. R. Gleitman and A. K. Josh, editors,Proceedings of the 22nd Annual Conference of theCognitive...

Báo cáo khoa học: The interaction between casein kinase Ia and 14-3-3 is phosphorylation dependent pot

... (seeMaterials and methods) for 90 min, and then incubated with and without NaF for anadditional 30 min at 30 °C before beingtested for interaction with 14-3-3. Lanes 1 and 2, 1% of the lysate used for ... implications for the respective roles of centaurin-a and the 14-3-3 isoforms in the regulation of signallingthrough CKI isoforms.Materials and methodsMaterialsAll chemicals and reagents were ... g and c isoforms (Fig. 2A). Den-sitometry analysis of the blot was performed to deter-mine the binding levels between the 14-3-3 isoforms.The amount of CKIa pulled down by equal amountsof...

Báo cáo khoa học: The predominant protein arginine methyltransferase PRMT1 is critical for zebrafish convergence and extension during gastrulation pdf

... for prmt1 morphants. (A) Dorsal view of embryos (10-somite stage) for krox20 staining.The positions of r3 and r5 are indicated. The widths of r3 and r5 and the vertical distance between r3 and ... to form the precursors of the meso-derm and endoderm, and C ⁄ E, in which cells accumu-late on the dorsal side and lead to axis formation.Gastrulation begins at 50% of epiboly (6 hpf) and ends ... cytoplasmicdomain of interferon-a receptor [11], and the putativemethylation of signal transducer and activator of tran-scription 1 (STAT1) [12–14] and protein inhibitor of activated STAT1...

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