... al. P. Robakowski et al.Temperature and photosynthesis in silver fir Original article Temperature response of photosynthesis of silver fir (Abies alba Mill. ) seedlings Piotr Robakowski a , Pierre ... be calculated) of the param- eters describing temperature responses of needle photosynthetic capacity in 4-year-old seedlings of silver fir (Abies...
Ngày tải lên: 08/08/2014, 14:20
... include: (a) the suppression of shivering thermogenesis; (b) a shift to a lipid-based metabolism; (c) the conservation of muscle glycogen stores for use during arousal, and (d) the stability of plasma ... slope ()0 . 8). The tem- perature at which phosphate had no allosteric effect was 6.6 ± 0.04 °C(n ¼ 4). However, when the enzyme was assayed in the presence of inhibitory level...
Ngày tải lên: 19/02/2014, 16:20
Báo cáo khoa học: Proteolytic activation and function of the cytokine Spatzle in the innate immune response of a lepidopteran insect, Manduca sexta ppt
... This resulted in insertion of a 10 amino acid segment (TREIDYPETI) and one substitution (Ser fi Gly) at the C-terminal end of the insertion (Fig. 1B). To examine the origin of the two proSpa ¨ tzle-1 ... activity, 100 lg of purified proSpa ¨ tzle-1A (20 ngÆlL )1 ) was activated as described above, diluted in 10 mL of 20 mm Tris ⁄ HCl and 200 mm NaCl (pH 8. 0), and then incub...
Ngày tải lên: 06/03/2014, 09:22
Báo cáo khoa học: Identification and characterization of 1-Cys peroxiredoxin from Sulfolobus solfataricus and its involvement in the response to oxidative stress pdf
... and C49S (m) was measured over a range of concentrations (0– 100 lgÆmL )1 ). (B) Effect on protection against DNA cleavage of the replacement of Cys 49 of rBcp2 with serine. Lane 1, pUC19; lane ... (CLUSTAL W 1.8 2) of Bcp2 from S. solfataricus and Prxs from A. pernix (APE227 8), T. maritima (Q9WZR 4), P. horikoshii (PH121 7), and human (PRDX 6). 0 0,1 0,2 0,3 0,4...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Cellular response to unfolded proteins in the endoplasmic reticulum of plants pptx
... S2P. A B Fig. 1. Comparison of the primary structure of ATF6 and Arabidopsis bZIP60 (A) and of yeast IRE1, Arabidopsis IRE1-1 (AtIre1- 1) and Arabidopsis IRE1-2 (AtIre1- 2) (B). The black bar represents ... localization of AtbZIP60 will probably clarify these issues. Orthologs of IRE1 have been identified in Arabidop- sis (AtIre1-1 and AtIre1- 2) and rice (Oryza sativa) (OsI...
Ngày tải lên: 16/03/2014, 11:20
Báo cáo khoa học:The isolation and characterization of temperature-dependent ricin A chain molecules in Saccharomyces cerevisiae docx
... molecules) R cryst b 0.175 (0.32 3) 0.173 (0.21 0) Reflections used 42 077 (292 8) 58 150 (388 4) R free c 0.209 (0.37 2) 0.197 (0.25 5) Reflections used 1779 (10 8) 2448 (17 0) R cryst (all data) b 0.177 ... r(I) 42.8 (9. 5) 41.6 (6. 8) R sym a 0.042 (0.07 8) 0.033 (0.11 4) Completeness ( %) 99.2 (96. 3) 91.6 (82. 2) Refinement Non-hydrogen atoms 255...
Ngày tải lên: 23/03/2014, 07:20
Báo cáo khoa học: The calcium-binding domain of the stress protein SEP53 is required for survival in response to deoxycholic acid-mediated injury pdf
... indi- cated combination of (B) fixed concentrations of DCA over the indicated time (500 l M), (C) increasing concentrations of ethanol (for 6 h), or (D) combination of fixed DCA (500 l M) and increasing ... (200 6) 1930–1947 ª 2006 The Authors Journal compilation ª 2006 FEBS the K d value. The log of the [Ca 2+ ] free (x-axis) is plotted against the log of bound ⁄ free dye {(R...
Ngày tải lên: 23/03/2014, 10:21
Báo cáo khoa học: Temperature and concentration-controlled dynamics of rhizobial small heat shock proteins doc
... different concentrations of HspB or HspB(G116A) was recorded at 360 nm. The CS assay was performed in the absence (r) and presence of 150 n M (n), 300 n M (m), 600 n M (h)and1.2l M (j)ofHspBpro- teins. ... Temperature-dependent dissociation and chaperone activity of wild-type HspB compared to HspB(G116A). (A) Gel filtration profiles of HspB (– ) and HspB(G116A) (- - -). The molecula...
Ngày tải lên: 23/03/2014, 12:20
Báo cáo khoa học: Temperature and salts effects on the peptidase activities of the recombinant metallooligopeptidases neurolysin and thimet oligopeptidase pdf
... XIII, cleavage site is L–R. Number X TOP (24.1 5) Neurolysin (24.1 6) k cat (s )1 ) K m (l M ) k cat /K m (l M )1 Æs )1 ) k cat (s )1 ) K m (l M ) k cat /K m (l M )1 Æs )1 ) L–R R–R R–R R–X I R – 11 1.5 7.3 ... system: (A) trifluoroacetic acid/H 2 O (1 : 100 0) and (B) trifluoroacetic acid/acetonitrile/H 2 O (1 : 900 : 10 0). The column was...
Ngày tải lên: 23/03/2014, 21:21
Báo cáo khoa học: "The response of dehydrated Douglas fir (Pseudotsuga menziesii) pollen to three in vitro viability assays and their relationship to actual fertility" doc
... examples of the 4 classes of germinating Douglas fir pollen. Figure 2 shows the average germination response (by class) of 8 Douglas fir pollen lots in each of 4 media ... FCH (198 7) Test of the first- on, first-in pollination hypothesis in coastal Douglas fir. Can J For Res 17, 63-68 Webber JE, Bonnet-Masimbert M (198 9) Influ-...
Ngày tải lên: 08/08/2014, 23:22