Báo cáo khoa học: "Comparison of the age-related porcine endogenous retrovirus (PERV) expression using duplex RT-PCR" ppt

... underscoring the importance of the interactions between the Tyr OH and the side chains of Asn174 and Asp148 in TEV protease (Fig. 4A). The importance of these interactions is fur- ther supported by the ... effects of the mutations. Hence, the results are consistent with the predicted role for these residues (based on crystal structure) in the interaction with subst...

Ngày tải lên: 19/02/2014, 16:20

... B. The receptor exposure is shown by the size and intensity (the darker the color, the more exposed the residue) of the disks drawn behind some of the residues to denote the difference in their ... B. The receptor exposure is shown by the size and intensity (the darker the color, the more exposed the residue) of the disks drawn behind some of the residu...

Ngày tải lên: 29/03/2014, 22:21

... difference in expression levels. The differences among the each age Comparison of PERV expression using duplex RT-PCR 319 Fig. 1. Sensitivity of the duplex RT-PCR detecting PERV g a g RNA and porcine ... determine the sensitivity of duplex RT-PCR, RNA was Comparison of PERV expression using duplex RT-PCR 321 group were observed in the lung, liver, and...

Ngày tải lên: 07/08/2014, 23:22

... postulated in the experiment of Fig. 2. Mutation of the essential amino acid Ser237 Establishment of the E. coli expression system of GluV8 enabled the roles of certain amino acids com- prising the protease ... propeptide of subtilisin adopts an arranged structure only in the presence of the mature form of the protease [23]. Whether or not a similar mechanism...

Ngày tải lên: 16/03/2014, 06:20

... sequences at both their ends. The study shows that the presence of flanking bases affects the thermodynamic stability of the G-quadruplex. With an increase in the flank length, the increase in the more favorable ... by the G-quadruplex in the presence of complementary strands using the quadru- plex-forming sequence located in the promoter region of the c- kit o...

Ngày tải lên: 07/03/2014, 02:20

... on both the pres- ence of a hydrophobic patch and the capacity of the protein to bind LPS. An understanding of the molecular mechanism that is responsible for the high binding affinity of antimi- crobial ... a result of the high number of lysines present in the structure, is dis- tributed uniformly on the protein surface. The absence of hydrophobic patches may b...

Ngày tải lên: 22/03/2014, 21:20

... impact in many areas of parasitology, including the identification and classification of parasites, the diagnosis of infections, the epidemiology of parasites, the analysis of population genetic ... 5.0 3.3 The use of the PCR method for identification of trematodes with the NC2- NC5 primer pair The results from testing eye flukes by the PCR method are...

Ngày tải lên: 06/08/2014, 19:20

... mea- surements. The thermopile was composed of four thermojunctions in series, located on either side of a 2 mm thick rubber. The sheath conductance of the gauge is calculated ... the estimation of the transpiration depends on the accuracy of the differential temperature measurement. The thermocouples must be protected against direct...

Ngày tải lên: 08/08/2014, 23:22

... ultrastructure fit with their function by measuring the rate of gas exchange under the prevailing envi- ronmental conditions in the canopy. Materials and Methods The willow stand ... week during the growing season, so that it received a total of 150 kg of N/ha/season. The stand was watered regularly to assure that the plants were not...

Ngày tải lên: 09/08/2014, 03:24

... The RBE value of 14.6 for cell reproductive death is similar to values in the range of 5 to 15 pu blished for many other lines of cultured cells [45]. The RBE of 1 derived for the induction of ... source. The 11 MBq 241 Am-sourcewaslocatedatadistance of 50 mm underneath the dishes and the particles passed through 50 mm of helium and the thin mylar bottom of th...

Ngày tải lên: 09/08/2014, 09:20