... added to 200 µl of cell suspension(1 × 107 cells/ml). After standing for 10 min at RT, 1500 µl of solution B (Stock solution containing 500 mg of Trypsininhibitor and 100 mg of RNase A/L, ... added.After incubation for 10 min at RT, 1000 µl of ice-cold solu-tion C [Stock solution containing 416 mg of PropidiumIodide (PI) and 1160 mg of Spermine tetrahydrochloride]was added. The solutions ... significant increase of relativeproportion in diploid and tetraploid cells (p<0.01), as com-pared to that of the control.Discussion On the basis of DNA contents, four main germ cellpeaks including...