... discovery of a specific
post-translational cleavage of recombinantly expressed
nitrilase from R. rhodochrous J1 which leads to the for-
mation of stable, active helices. 3D reconstruction of
the negatively ... Yamamoto M,
Kumasaka T, Ueki T, Nanba H, Ikenaka Y, Takahashi
S, Sato M et al. (2000) Crystal structure of N-carbamyl-
d-amino acid amidohydrolase with a...
... background of at least
1000 subjects was typical of the American Midwest with
90% of European ancestry and 5% each of African
and Asian ancestry. An exception was the targeted analysis
of ... its altered N-terminal truncation
on the metabolic functions of ApoC1.
Abbreviations
ApoC1, apolipoprotein C1; ApoC2, apolipoprotein C2; ApoC3-0, ApoC3 that does not contain a carb...
... Journal 272 (2005) 45494557 ê 2005 FEBS
Two novel variants of human medium chain acyl-CoA
dehydrogenase (MCAD)
K364R, a folding mutation, and R256T, a catalytic-site mutation
resulting in a well-folded ... Medium chain acyl-CoA dehydrogenase (MCAD) is a
homotetrameric flavoprotein that catalyses one of the
recurrent steps in t...
... Gdm-HCl-induced unfolding;
Escherichia coli; molten globule; protein
unfolding intermediates of IbeA
Correspondence
K. S. Kim, Division of Pediatric Infectious
Diseases, Johns Hopkins University School
of Medicine, ... is
available on protein folding intermediates [11–18],
there are no reports available for E. coli invasin pro-
tein IbeA. The process of unfolding a...
... domains. The largest domain encompasses strands b1
to b6 a nd strand b7 to the C-terminus. The central b sheet is
composed of 7 parallel b strands associated to an anti-
parallel strand (b2) and is ... of Rv1399c from M. tuberculosis (Eur. J. Biochem. 271) 3955
Expression and characterization of the protein Rv1399c
from
Mycobacterium tuberculosis...
... protein
sequencer.
Results
Purification of aspartate racemase from
B. bifidum
The aspartate racemase was purified to homogeneity from
cell extracts of B. bifidum NBRC 14252. A summary of the
purification is presented ... Linear alignment of amino acid se-
quences of PLP-independent aspartate race-
mases. Bb, the monomeric aspartate racemase
from B. bifidum; St,...
... no-
table since the Google model only requires n-gram
counts to implement. The Backoff Erk model is
the best, using the Baseline for the majority of
decisions and backing off to the Erk smoothing
model ... is the concatenation of two
words (e.g. house/car). One word is the orig-
inal in a document, and the second is the con-
founder. Consider the following example...
... hydrogen peroxide reversibly inhibits
protein-tyrosine phosphatase 1b in vivo and enhances
Calpain-mediated degradation of reversibly oxidized protein-tyrosine phosphatase 1B A. Tru
ă
mpler et ... fragment, consistent with the lack of a part of the catalytic core (see Fig. 3).
Calpain-mediated degradation of reversibly oxidized protein-tyrosine phospha...
... downshift of the 4CẳO stretching vibration of
Q
A
by 60 cm
)1
[10,11], indicating strong asymmetric
binding of UQ
10
at the Q
A
site, in contrast to symmetric,
weaker binding of UQ
10
at the Q
B
site ... reaction
centre of Rb. sphaeroides should provide the methoxy
orientations of UQ
10
at the Q
A
and Q
B
binding sites.
However, there are a large...
... the
change of the molecular species composition of the cel-
lular Gb
3
[28,29]. In in vitro binding assays, the fatty
acid chain of Gb
3
was found to in uence the binding
to Shiga toxin [30,31].
In this ... clathrin function
has minimal effects on Shiga toxin endocytosis, show-
ing that Shiga toxin can enter cells efficiently via clath-
rin-independent endocytic mechan...
... is different from that of human,
mouse and other reported fish hepcidins. In liver, some
cells expressed both types of hepcidins, while other
cells expressed only one of them or neither of them
(Fig. ... plated on the appropriate agar plates
and incubated for 24 h.
Hemolytic activity of synthesized Japanese floun-
der hepcidins
The hemolytic activity of the synthet...
... +82-53-950-5975; Fax: +82-53-950-5955
E-mail: jeongks@knu.ac.kr
Two different types of malignant fibrous histiocytomas from pet dogs
Sun Hee Do
1
, Il-Hwa Hong
2
, Jin-Kyu Park
2
, Ae-Ri Ji
2
, ... are indeed from a primitive cell
type or are the end result of “differentiation” of several
different types of sarcomas [4,8]. The undifferentiated
mesenchymal cell-or...
... rotation of F(1)-ATPase
during catalysis. Proc Natl Acad Sci 2001, 98(24):13649-13654.
20. Paik SR, Yokogama M, Yoshida M, Ohta T, Kagawa Y, Allison WS:
The TF1-ATPase and ATPase Activities of Assembled ... Indiana, USA). Other chemicals were of the
highest grade commercially available.
Preparation of F1-ATPase
The α
3
β
3
γ core complex of F
1
-ATPase originating from
Bacillus PS3...
... hexokinases have lost intron 2,
which is present in the other moss hexokinases, includ-
ing the type D hexokinases (Figure 1). Finally, we note
that the sequence of the type C hexokinase, PpHxk4, is
more ... of the Physcomitrella hexokinases
We proceeded to study the intracel lular locations of the
moss hexokinases. Sequences from the new hexokinases,
e...