... each of the two lateral lobes. During spermatogenesis, mature spermatozoids accumulate and are maintained in vas deferens until fertilization, which results in a dramatic size increase. In the ... of protein of the [ 3 H]hippurate obtained after incubation with and without 10 l M captopril. Soluble (SOL) and insoluble (INSOL) fractions were tested in animals in active sp...
Ngày tải lên: 30/03/2014, 01:20
... PT, Binns MM. Insertion of the fusion gene from Newcastle disease virus into a non-essential region in the terminal repeats of fowlpox virus and demonstration of protective immunity induced ... T. Characterization of haemagglutinin-neuraminidase glycoprotein of Newcastle disease virus expressed by a recombinant baculovirus. Virus Res 1991, 20, 31-43. 23. Of...
Ngày tải lên: 07/08/2014, 20:24
Tài liệu Báo cáo khoa học: Synchronization of Ca2+ oscillations: a coupled oscillator-based mechanism in smooth muscle ppt
... exacerbated when one considers that millions of cells, each with its own intrinsic rhythm, partici- pate in this ‘mob action’, and yet a meaningful global outcome emerges. It is fascinating that in ... cou- pling, in that store-generated changes in [Ca 2+ ] c are required to activate inward membrane current, with the resulting membrane depolarization activating or advancing the ph...
Ngày tải lên: 16/02/2014, 09:20
Tài liệu Báo cáo khoa học: Phosphorylation of hormone-sensitive lipase by protein kinase A in vitro promotes an increase in its hydrophobic surface area ppt
... including bis-ANS, the reaction rates were constant for at least 30 min of incubation at 37 °C. HSL phosphorylation using 32 P-labelled ATP HSL (9 lg) was phosphorylated at room temperature in 110 ... ATP concentrations with TO as substrate (Fig. 4B). Incubatation of the enzyme preparations with alkaline phosphatase did not affect the enzymatic activity (data not shown). This indi...
Ngày tải lên: 18/02/2014, 11:20
Tài liệu Báo cáo khoa học: Delineation of exoenzyme S residues that mediate the interaction with 14-3-3 and its biological activity ppt
... activity in vivo. We have approached these questions using a strategy of single amino acid site-directed mutagenesis of the cofactor interaction domain within ExoS. Various single mutant ExoS proteins ... for phosphorylation-independent binding The interaction between 14-3-3 and ExoS is important for the ADP ribosylation activity of ExoS and even more intriguingly, appears to be i...
Ngày tải lên: 19/02/2014, 08:20
Tài liệu Báo cáo khoa học: Integrase of Mason–Pfizer monkey virus doc
... evident after 1 min of incuba- tion and was linear for 15 min at 37 °C. The analysis of M-PMV integrase integration activ- ity confirmed that joining of substrates catalyzed by M-PMV integrase is ... [17]. Retroviral integrases contain two known metal-bind- ing domains. The N-terminal domain includes a zinc- finger motif and the central catalytic core domain contains a triad of ac...
Ngày tải lên: 19/02/2014, 16:20
Tài liệu Báo cáo khoa học: Dissociation of DNA polymerase a-primase complex during meiosis in Coprinus cinereus pptx
... further separated by SDS/ PAGE and incubated with DNA substrate during a renaturation process, indicates that the catalytic core of DNA polymerase activity resides in the 140 kDa protein molecule ... m M MgCl 2 ,5m M dithiothreitol, 2 m M ATP, 80 gÆmL )1 of poly(dT), 20 l M dATP, 4 lCi of [a- 32 P]dATP (6000 CiÆmmol )1 ), and 4 lL of purified fraction. Incubation was perfomed at...
Ngày tải lên: 20/02/2014, 11:20
Báo cáo khoa học: Interaction of the general transcription factor TnrA with the PII-like protein GlnK and glutamine synthetase in Bacillus subtilis potx
... C) Effects of AMP, glutamine and ATP on GS binding to TnrA. GS was preincubated with effector molecules at the concentrations indicated, and injected onto the His 6 -TnrA surface. GS incubated in pure ... from S. elongatus was injected into FC 1 in a volume of 50 lL at a concentration of 2 nmol ⁄ mL in HBS buffer. This resulted in increases in resonance units of 500...
Ngày tải lên: 06/03/2014, 00:21
Báo cáo khoa học: Structure of the atrial natriuretic peptide receptor extracellular domain in the unbound and hormone-bound states by single-particle electron microscopy ppt
... containing an extracellular ANP- binding domain (ECD), a transmembrane domain, and an intracellular domain consisting of an ATP-binding regulatory domain and a GCase catalytic domain [8]. ANP binding ... domains in the opposite direction without an appreciable change in the interdomain distance. (B) Schematic presentation of the movement of the juxta- membrane domains in respo...
Ngày tải lên: 07/03/2014, 03:20