... and bundling triggered byhhLIM and CRPs requires, in the first instance, identi-fication of their actin-binding domains. To date, none of the actin-binding domain sequences registered in databases ... includedSM22a, a known actin cross-linking protein, and BSA,which does not interact with or cross-link actin. In the absence of hhLIM, the majority of actin remained in the supernatant (S) and ... the second LIM domain plays a central role in bundling of F-actin. Taken together, these data identify hhLIM as an actin-binding protein that increases actin cytoskeleton stability by promoting...