... libraryusing a combination of PCR amplification, mass sequencing and library screening as described in the Materials and methods. The first clone was 1689 bp and contained anORF of 1434 bp coding for ... R.R.L. (1975) Culture of phytoplankton for feedingmarine invertebrates. In Culture of Marine Invertebrate Animals(Chanley, M.H., ed.), pp. 29–60. NY. Plenum Press.24. Shanklin, J. & Cahoon, ... biosynthesis. Using a combination of PCR, mass sequencing and library screening, the codingsequences of two desaturases were identified. Both proteinsequences contained a cytochrome b5domain fused...