... s-DAPK-1), which shares some domains with full-length DAPK-1: from part of the ankyrin-repeatregion, through to part of the cytoskeleton bindingdomain, and concluding with a unique tail extensionof ... s-DAPK-1Dtail with a 42amino acid tail deletion is named Flag-TD. (B–D) Transfected s-DAPK-1 and its mutants identified a cleavage within its tail. HCT116p53+ ⁄ +cells were transfected with the ... twice with NaCl ⁄ Piand then scraped into1 mL of NaCl ⁄ Pi. The HA–DAPK-1 was a kind gift from A. Kimchi (Weizmann Institute, Rehovot, Israel). s-DAPK-1 was cloned into the Flag–Myc vector from...