Báo cáo khoa học: " Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice" pptx

... S. cerevisiae vector control. Group C: Oral vaccination with S. cerevisiae expressing ApxIA antigen. Grou p D: Oral vaccination with S. cerevisiae expressing ApxIIA antigen. Group E: Combined oral ... immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA...

Ngày tải lên: 07/08/2014, 20:23

... the induction of pro-inflammatory cytokines and granulocytes into the lungs following either intramuscular or intranasal VLP vaccination. The recombinant influenza virus-like particle vaccines preserve ... elicited by vaccinationFigure 1 Antibodies elicited by vaccination. Mice (n = 8) were vaccinated via intramuscular injection at weeks 0 and 3 with VLPs (clade 1 or clade...

Ngày tải lên: 20/06/2014, 01:20

... N-terminal domains (N1, N2) of the minor coat protein (g3p) are responsible for binding and infection in E. coli.Thus, incorporation of a library of target proteins between the N-terminal domain ... Protein design by binary patterning of polar and non- polar amino acids. Science 262, 1680–1685. 8. Michnick, S.W. (2001) Exploring protein interactions by inter- action-induced fold...

Ngày tải lên: 19/02/2014, 12:20

... use of leucine, isoleucine, valine, methionine, tyrosine, tryptophan or phenylalanine as a sole nitrogen source, leads to the formation of the fusel alcohols and acids (Fig. 1) [1]. Indeed, in Saccharomyces ... of N-terminal methionine from recom- binant proteins by engineered E. coli methionine amino- peptidase. Protein Sci 13, 1802–1810. 29 Duggleby RG (2006) Domain relationsh...

Ngày tải lên: 06/03/2014, 00:21

... Japan 2 Laboratory of Biopharmaceutical Research, National Institute of Biomedical Innovation, 7-6-8 Saito-asagi, Ibaraki, Osaka 567-0085, Japan 3 The Center for Advanced Medical Engineering and Informatics, ... such as nSP30 would induce Th2-polarized allergic immunity. Our results support previous reports showing that the immune- activating effect of nanomaterials increases with de...

Ngày tải lên: 21/06/2014, 05:20

... an important role in protection of neuronal cells against stress-induced apoptosis [26]. In accordance with these findings, ischemia/reperfusion in the rat forebrain induces the expression of HSP105/110 ... activation of p38 induced by oxidative stress, we further examined whether its activation is responsible for the induction of apoptosis in F9 cells using SB202190, a pot...

Ngày tải lên: 21/02/2014, 03:20

... studied by means of capillary e lectrophoresis combined with laser-induced fluorescence detection, confo- cal laser scanning microscopy and fluorescence-activated cell sorting. Incubation with the fluorescein-labelled ... , respectively. Results Conjugation with MAP leads to an increased intracellular availability of PNA In order t o e xamine the ability of M AP to de live r PNA...

Ngày tải lên: 23/03/2014, 13:20

... alkylating agents by linking the alkylating pharmacophore to the DNA-affinity molecules (e.g., DNA intercalating agents, DNA minor groove binder) [7,8]. In most cases, the DNA-directed alkylating ... irradiated with 2 Gy of g-rays. The Annexin V/PI staining was then determined with FACS analysis. Cells treated with either 200 nM of BO-1051 alone or combined with irradiation...

Ngày tải lên: 09/08/2014, 09:20

... a-synuclein. For this, a-synuclein was incubated in the presence of MPTP and MPP + along with dopamine. The fibrillation of a-synuclein was monitored by Thioflavin T fluorescence and immunoblotting. ... occur in the brain include selective loss of dopaminergic neurons in substantia nigra pars compacta and appearance of Lewy bodies consisting of aggregated protein, mainly a-sy...

Ngày tải lên: 14/02/2014, 19:20

... inhibitors, binding revers- ibly into the active site of the enzyme. Cystatins, including human cystatin C, are general inhibitors of cysteine proteases. For cathepsin B, their K i value is in nanomolar ... mech- anism applies to the binding of cystatin C to cathepsin B, which takes place in two steps: an initial weak inter- action with N-terminal region of the inhibitor inducin...

Ngày tải lên: 18/02/2014, 11:20