... for the optimal activity of BsArb43B.Furthermore, to determine whether the role of the calcium is structural, thermal shift assays were per-formed to determine the Tm of the protein in the ... distribution of BsArb43B in the proximity of the active site. Arabinotriose in the binding cleft is representedusing sticks. (B) Detailed view of the binding cleft with arabinotri-ose. (C) Details of the ... 45624574 ê 2010 The Authors Journal compilation ª 2010 FEBS 4563 New evidence for the role of calcium in the glycosidase reaction of GH43 arabinanases Daniele de Sanctis1,2,*, Jose´M. Ina´cio1,*,,...