Báo cáo khoa học: "Primary structure of mature SAG1 gene of an Indonesian Toxoplasma gondii and comparison with other strains" pps

Ngày tải lên: 07/08/2014, 18:21

... nitrogen nuclei both of 15 NO and of 14 N-proximal ligand cause the splitting. The heme proximal ligand is considered to be His16, which corresponds to His17 of the proximal ligand of heme- Syn HO-1. ... characterization of the cyanobacterial HO-2 protein and its heme complex. Results and Discussion Expression and purification of Syn HO-2 Culturing the cells at two temperat...

Ngày tải lên: 30/03/2014, 15:20

... Kruus 2 , Juha Rouvinen 1 and Nina Hakulinen 1 1 Department of Chemistry, University of Eastern Finland, Joensuu, Finland 2 VTT Technical Research Centre of Finland, Espoo, Finland Keywords ascomycete; ... b-bar- rels of domains A and B (Fig. 2B), and had six hydro- gen bonds, three to the main chain carbonyls at Asn6, Leu168, and Val170, and three to the side chains of Ar...

Ngày tải lên: 14/02/2014, 18:20

... Hospital and University of New South Wales, Sydney, NSW, Australia 4 School of Chemistry and Molecular Biosciences and Centre for Infectious Disease Research, University of Queensland, Brisbane, ... importin-a recognition of the NLS and is conserved in all human CLICs, except CLIC3. The significance of the motif is clear, with 92% of the hydrogen bonds formed between impo...

Ngày tải lên: 14/02/2014, 19:20

... (Fig. 3A). Three of the ligands, OD2 of Asp165, NE2 of His79 and NE2 of His169, formed an equatorial plane. The other protein ligand, NE2 of His31, bound to the metal, in the company of a water oxygen, ... coordi- nated with six ligands: the five same ligands in the Mn-reconstituted enzyme and an additional water oxy- gen, which, together with the OD2 of Asp165, the...

Ngày tải lên: 14/02/2014, 22:20

... crystal structures of the ligand-free PhyK at 1.7 A ˚ resolution and of a cata- lytically incompetent PhyK mutant that suggest a model for substrate binding. Comparison with the structures of the ... compilation ª 2010 FEBS mutagenesis of phyK. The H25A mutation was confirmed by sequencing analysis. Gene expression and purification of PhyK The genes encoding PhyK and the m...

Ngày tải lên: 16/02/2014, 09:20

... determined the structure of HtA by NMR methods. The structure consists of one a-helix, a helical turn and seven b-strands that form an N-terminal hairpin and an anti-parallel b-sheet, with a characteris- tic ... FEBS involvement of a catalytic pair constituted by an acid and a base on each side of the hydrolyzed bond [16]. E96 and H137 in a-sarcin and E58 and H...

Ngày tải lên: 18/02/2014, 08:20

... shows excellent stereochemistry, with 98.5% of all residues in the most favored and favored regions of the Ramachandran plot and r.m.s.d. values for bond and angle of 0.005 A ˚ and 1.37 ° as shown in Table ... crystal structure of the complex of this protease with benzamidine. Results and Discussion Overall structure The catalytic domain of DESC1 resembles an o...

Ngày tải lên: 19/02/2014, 00:20

... hydrogen-bon- ded antiparallel b-strands (b6–b7), an a-helical hairpin (helices a5, a6) and a C-terminal strand (b8). Helix a5 and strand b7 partially cover one side of the Ross- mann fold structure, ... three-stranded b-sheets, each one consisting of the antiparallel b-strands b6, b7 of one monomer and strand-b8 of the other. Solvent-accessible surface [15] calculations sho...

Ngày tải lên: 19/02/2014, 00:20

... B area, protection of the nucleosome C area and repositioning of initially randomly positioned nucleosomes (Fig. 5A and B, compare lanes 1, 2, and 3, 4 and corresponding scans). Quite unexpectedly, ... TSA administration has on chromatin structure and on gene expression. Materials and methods DNA and plasmids Construction of the MMTV reporter and the plasmid for in v...

Ngày tải lên: 19/02/2014, 12:20