Báo cáo khoa học: "Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus" pps

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Ngày tải lên: 07/08/2014, 18:21

... alain.hamon@univ-angers.fr Abbreviations: Aah2, alpha toxin II from the venom of the scorpion Androctonus australis hector, also called AaH; ATX II, toxin II of the sea anemone Anemonia sulcata; Bom3,4, a- like toxins from ... mass of Lqh6 and the calculated molecular mass indicates amidation of the C-terminal amino acid residue. The absence of a free a- carboxyl...

Ngày tải lên: 17/03/2014, 11:20

... instructions (Amersham Pharmacia). The amount of cell lysate incubated with the beads was adjusted so that a similar amount of each subunit was bound to the beads. The beads were washed once and then ... Identification of cDNA clones for the large subunit of eukaryotic translation initiation factor 3. Comparison of homologues from human, Nicotiana tabacum, Caenorhabditi...

Ngày tải lên: 23/03/2014, 21:20

... Kishida, Y., Kohara, M., Matsumoto, M., Matsuno, A. , Muraki, A. , Nakazaki, N., Siumpo, S., Sugimoto, M., Taka- zawa,M.,Yamada,M.,Yasuda,M.&Tabata,S.(2001)Com- plete genomic sequence of the filamentous ... basis of the highly reversible photochemistry of a- PEC and the protein dynamics related to the transformation, the other is to evaluate the potential of the re...

Ngày tải lên: 21/02/2014, 03:20

... isolated previously [22] using the following primers: gatggatcccatATGGGTG TTGAAGTTGTA annealing around the start codon of the Ppi1 ORF and gactcgagATTAGTCGACTTCTTACGC annealing just before the putative ... with antisera against the N-terminus of PPI1 (A) or the C-terminus of the H + -ATPase (B). His 6 –ACA8(1–116) reproduces the N-terminus of an A. thaliana PM Ca 2+...

Ngày tải lên: 19/02/2014, 07:20

... 5¢-TTGATCGATTCTGTCTATGCCC CA-3¢ along with the adaptor primers: AP1 (5¢-GTAATAC GACTCACTATAGGGC-3¢) and AP2 (5¢-ACTATAGGG CACGCGTGGT-3¢). Nested PCR was carried out in a final volume of 50 lL containing 1 lL of a genome ... 5¢-GATGAAAATCCTAACCTCTCCCCCGCACAG- 3¢; OP7, 5¢-ACTGCACCTACGGCGGGTCGTTGGTACG TG-3¢; NP4, 5¢-GACACCGTAGGTTGAGCCGCCAATC GTCCC-3¢; NP5, 5¢-CTTTAACTTGTTGGGCACTGG CATTG...

Ngày tải lên: 19/02/2014, 07:20

... pGL3e:Prm 3a AP)1 *, pGL3b:Prm3ab AP)1 *, pGL3e: Prm3ab AP)1 *, pGL3b: Prm3aa AP)1 *, pGL3e:Prm3aa AP)1 *, pGL3b:Prm3aaa AP)1 * and pGL3e:Prm3aaa AP)1 *. Mutation of the Oct-1 element with the sequence aaA TGCa to aaTTCCa ... to aaTTCCa (core bases shown in uppercase letters) centered at )123 within Prm3 was performed using the mutator primers Kin175 (5¢-CACCAGAGCTACTTACA CTGAATTCCAG...

Ngày tải lên: 19/02/2014, 16:20

... native signal peptide was amplified by PCR using the following primers: 5¢ primer, 5¢-GAGCCCGGATCCACCATGAA GGTCTCAATAATT 3¢;3¢ primer, 5¢-CTGACG GAAT TCTTAAACATTAATGCC 3¢. These primers encoded a Kozak ... W81, as observed with M. brassicae CSPMbraA6 [32]. We observed also that BrC15-Ac was able to displace ASA, suggesting that brominated fatty acid and ASA both associated with W81 in the...

Ngày tải lên: 21/02/2014, 03:20

... the Bradford assay using BSA as standard. Assay The decarboxylation activity of ScPPDC-His on a range of aromatic and aliphatic 2-keto acids was monitored at 30 °C by a coupled assay described ... A com- parison of these and the parameters of the wild-type enzyme are presented in Table 3. ScPPDC-E545L was unstable in the buffer used for the IPyA assay and thus was as...

Ngày tải lên: 06/03/2014, 00:21

... member of the CCD1 subfamily was identified from Arabidopsis thaliana [26], and was later shown to act as a dioxygenase [27]. Sequence homology then allowed the identification and charcterization of ... low amount of the C 14 dialde- hyde is probably a result of instability. The formation of b-ionone from b-apo-8¢-carotenal (C 30 ) and b-apo- 10¢-carotenal (C 27 ) sugg...

Ngày tải lên: 07/03/2014, 03:20