Báo cáo khoa học: "Characterization of Chinook head salmon embryo phenotypes of infectious salmon anemia virus by real-time RT-PCR" docx

Ngày tải lên: 07/08/2014, 18:21

... 10 6 mL )1 . Next, 0.5 mL of propidium iodide staining solution (10 mg of propidium iodide, 0.1 mL of Triton-X, 3.7 mg of EDTA in 100 mL of NaCl ⁄ P i ) was added to 0.5 mL of cell suspension. The ... the onset of apoptosis is accompanied by an increase in ML intensity [5–7], although other studies have not [8,9]. A number of studies have been performed in view of the poss...

Ngày tải lên: 18/02/2014, 13:20

... mean + SE of three pools of four animals (tissues) or the mean of one pool of embryos or larva from one spawn. Expression levels are related to 100 copies of GAPDH. (B) Localization of Cg-Clp2 ... development of the gills and the production of an adult shell), which is accom- panied by significant growth of the soft body parts [21]. The mantle edge governs shell formation a...

Ngày tải lên: 19/02/2014, 00:20

... Formation of the up-shifted complex, indicated by the arrow, was decreased by the addition of compet- ing unlabeled self oligonucleotide or Sp1 oligonucleo- tide (lanes 2 and 4), but not by addition of oligonucleotide ... serum medium, followed by mixture with 6 lL of Lipofectamine Plus reagent at room temperature for 10 min. Four microliters of Lipofectamine Plus reagent we...

Ngày tải lên: 19/02/2014, 06:20

... action of the enzyme C-terminus, but further enhances the activity of the enzyme whose C-terminus has been displaced by low pH or by FC-induced binding of 14-3-3s. Results The C-terminus of isoform ... conformation determined by cleavage of the C-terminus [1,9–16]. Also, blue-light activation of PM H + -ATPase in guard cells of broad beans involves protein kinase-mediated...

Ngày tải lên: 19/02/2014, 07:20

... were inhibited by addition of SDS dur- ing activity measurements, and showed  30% of the maximum activity in presence of 1% SDS (Table 2). The effect of EDTA on activity was measured by including ... presence of denaturants like urea and SDS. Cleavage of protein substrates by PRK is in fact stimulated by SDS [21]. The enhanced activity in the presence of SDS is probably...

Ngày tải lên: 19/02/2014, 07:20

... of all of these genes remains to be clarified. Expression and purification of Sno1p and Snz1p In light of the similarity in the amino acid sequence of Sno1p to that of the glutaminase subunit of ... verification of the structure Fig. 6. Inhibition of the glutaminase activity of the complex of Sno1p with a His-tag and Snz1p by 6-diazo-5-oxo- L -norleucine (DON). The reacti...

Ngày tải lên: 19/02/2014, 12:20

... differences between the TP isoforms in terms of their mechanisms of signaling [18,19], modes of regulation⁄ desensitiza- tion [20–23] and patterns of expression [24], this repre- sents a question of potentially ... (2002) Characteri- zation of the 5¢ untranslated region of alpha and beta isoforms of the human thromboxane A2 receptor (TP). Differential promoter utilization by...

Ngày tải lên: 19/02/2014, 16:20

... from either a perfect shield of site B afforded by bilirubin, or, other- wise, by an alternative conformation of the bilirubin– bilitranslocase complex, totally missed by antibody B. Cyanidin 3-glucoside ... presence or in the absence of various concentrations of a ligand A, k 2 and k 1 are the rate constants of the inhibition of the bilitranslocase– bilirubin complex and o...

Ngày tải lên: 20/02/2014, 01:20

... Hiromi K (1970) Interpretation of dependency of rate parameters on the degree of polymerization of substrate in enzyme-catalyzed reactions. Evaluation of subsite affinities of exo-enzyme. Biochem Biophys ... reactions were stopped by cooling on ice and by addition of 0.4 sample volume of 50 mm NaOH, thereby increasing the pH to 8.0–8.5. Samples were stored at )20 °C until a...

Ngày tải lên: 20/02/2014, 03:20