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Ngày tải lên: 07/08/2014, 18:21
... M r values of the standards in thousands. Lanes 2–9, 5 lg of peak P I, P II, P III ⁄ P IV and a mixture of other isoforms (*), respec- tively, in the presence (lanes 2–5) or absence (lanes 6–9) of 2-mercaptoethanol. ... similarity of the A- and B-chains of the four isoforms, a pairwise alignment was performed and the values of identity expressed in Fig. 3. (Continued). Ch...
Ngày tải lên: 18/02/2014, 16:20
Báo cáo khoa học: Isolation and characterization of a D-cysteine desulfhydrase protein from Arabidopsis thaliana pptx
... lmol of pyruvate, 1.63 lmol of NH 3 , and 1.47 lmol of elemental selenium were produced from 0.75 lmol of d-selenocystine. When the reaction was carried out in sealed tubes in which air was displaced by ... sequences from three species are shown). The respective protein from Hansenula saturnus was already crystallized and a model of its 3D structure determined [19]. Intere...
Ngày tải lên: 16/03/2014, 18:20
Báo cáo khoa học: Isolation and characterization of an IgNAR variable domain specific for the human mitochondrial translocase receptor Tom70 potx
... degree of affinity and specificity for Tom70, andwithaK D of 2n M compares very well with affinities reported for camelid V H H domains (that vary in the range 2–300 n M [36]) and for scFv and disulphide ... paratope and assessment of the varying contributions of CDR and framework regions clearly requires definitive structural data; this analysis is currently in progress. The...
Ngày tải lên: 17/03/2014, 10:20
Báo cáo khóa học: Isolation and characterization of the Xenopus HIVEP gene family ppt
... combinations of degenerate and specific primers and by rescreening the cDNA libraries. The partial clones of XHIVEP2 and -3 covered 5.9 and 3.2 kb of the respective 3¢ ends and included 3¢-UTRs and poly(A)-tails. A ... component of the TGF-b signaling pathway. In this study, we describe the isolation of Xenopus HIVEP1,aswell as partial cDNAs of HIVEP2 and -3. Analys...
Ngày tải lên: 30/03/2014, 13:20
Báo cáo khoa học: " Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells" pps
... 4E and F). Discussion Isolation and characterization of stem cells derived from various tissues and sources have been one very critical issue for stem cell therapy [12,28,33]. The purpose of ... that Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells 183 Fig. 1. Identification of the cumulative population doubling level (C...
Ngày tải lên: 07/08/2014, 23:22
Tài liệu Báo cáo khoa học: Production and characterization of a secreted, C-terminally processed tyrosinase from the filamentous fungus Trichoderma reesei ppt
... (1996) Tyrosinase isoforms from the fruitbodies of Agaricus bisporus. Phytochemistry 43, 333–337. 11 Magnus KA (2001) Hemocyanins from arthropods and molluscs. In Handbook of Metalloproteins (Messersch- midt ... Buchert 1 and Kristiina Kruus 1 1 VTT Technical Research Centre of Finland, Espoo, Finland 2 Protein Chemistry Research Group and Core Facility, Institute of Biotech...
Ngày tải lên: 19/02/2014, 06:20
Tài liệu Báo cáo khoa học: Cloning and characterization of CBL-CIPK signalling components from a legume (Pisum sativum) ppt
... treatment of pea seedlings were cold (A and B), salinity (C and D), wounding (E and F), drought (G and H), SA (I and J), ABA (K and L) and calcium (N and O). Panel M is the control for ABA responsive ... D, I and L are diamidino-2phenylindole hydrochloride (DAPI) stained cells showing the nuc- leus, and Fig. 7C, F, K and N are the merged images of A and B, D and...
Ngày tải lên: 19/02/2014, 07:20
Tài liệu Báo cáo khóa học: Cloning and characterization of two distinct isoforms of rainbow trout heat shock factor 1 ppt
... 10.0; GAPEXT ,0.05; GAPDIST ,8; MAXDIV , 40; ENDGAPS ,off; NOPGAPS ,off; NOHGAPS ,off.Graphical output of the bootstrap figure was produced by the program TREEVIEW . Isolation of RNA and RT-PCR analysis Total RNA was isolated from RTG-2 cells and ... distinct HSF1 isoforms contribute to the tissue specificity of the heat shock response. Airaksinen et al. [7] have reported that the...
Ngày tải lên: 19/02/2014, 12:20
Báo cáo khoa học: Synthesis and characterization of a new and radiolabeled high-affinity substrate for H+/peptide cotransporters pdf
... mammalian peptide transporters derived from the intestinal and renal isoforms [10,11], site-directed mutagenesis experi- ments [12–14] and from extensive studies on substrate specificity combined ... limiting step of peptide transporters has still not yet been determined, and the number of transporters per cell and their turnover rate in epithe- lial cells is not known. With re...
Ngày tải lên: 07/03/2014, 05:20