Báo cáo khoa học: "Transcranial Doppler ultrasound analysis of resistive index in rostral and caudal cerebral arteries in dogs" pps

... RCA and CCA obtained from male and female were compared usingindependent t-test. The RI in RCA obtained from fontanellewindow and temporal window were compared usingindependent t-test. In order ... KoreaTranscranial Doppler (TCD) was carried out todetermine the resistive index (RI) values of normal canine cerebral arteries and its reproducibility and to evaluatethe change of cerebral vascular ... analysis of resistive index in rostral and caudal cerebral arteries in dogsMinho Seo, Hojung Choi, Kichang Lee, Mincheol Choi, Junghee Yoon*College of Veterinary Medicine, Seoul National University,...

Báo cáo khoa học: Semi-nested PCR analysis of unknown tags on serial analysis of gene expression potx

... transcription–PCR analysis of unknown serial analysis of geneexpression tags; rSAGE, reverse serial analysis of gene expression; SAGE, serial analysis of gene expression; TSAT-PCR, two-step analysis of unknown ... sequence downstream of the most 3¢NlaIII restriction site. In contrast, the remaining PCRproduct was a large size band of the no. 10 product, in which sequences of UP-II and oligo(dT16) were ... However, step 4 (linker liga-tion) in the rSAGE protocol does not avoid self-ligation of the cDNA, and the self-ligation would leadto smearing in the following PCR ampliﬁcation. In addition, the...

Báo cáo khoa học: Modular metabolic control analysis of large responses in branched systems – application to aspartate metabolism potx

... determined from data obtained bychanging the concentration of the linking intermediate and measuring the corresponding rates of the modules(i.e. from module rates versus S data). Changes in theintermediate ... effect of removing the inhibition of AKI and AKII, and theinhibition of HSDHI and HSDHII, separately. In bothprocedures, the corresponding maximal rates wereadjusted such that the starting values ... of the isoforms of monofunc-tional AK (AK1 and AK2) by Lys (G-III), inhibition of the isoforms of DHDPS (DHDPS1 and DHDPS2)by Lys (G-IV), and activation of TS (TS1) by AdoMet(G-V). In module...

Báo cáo khoa học: Antibody-based proteomics Analysis of signaling networks using reverse protein arrays pdf

... blockage of the ATP-binding site in the catalytic domain of the kinase. As the majority of kinase inhibitors that are discovered by high-throughput screening methods target the ATP-bindingpocket of ... exam-ple erlotinib (Tarceva) and geﬁtinib (Iressa), both of which block the epidermal growth factor receptortyrosine kinase. Tyrosine kinase inhibitors act byinhibiting the activation of intracellular ... aspects of pathway events and (d) the proﬁling of compounds to reveal signaling and cross-pathway effects of drug candidates. In addi-tion, analysis of healthy versus diseased tissue (includ-ing...

Báo cáo khoa học: "On-Line Semantic Analysis of English Texts" ppt

... derived inductively. Before the analysis begins, an initial set of functions breaks each sentence of a paragraph into strings of words, and, in certain circumstances, reforms discontinuous ... respectively: (1) KIND, KIND, GRAIN, and (2) FOLK, DO, GRAIN. Now the rules of template production permit both FOLK + DO + GRAIN and KIND + GRAIN in rank I, the latter by transposition and deletion ... damp." In British English that sentence allows two quite different interpretations: "a certain kind of human being is in a certain state," and "a certain kind of chemical...

Báo cáo khoa học: Modular metabolic control analysis of large responses The general case for two modules and one linking intermediate docx

... literature. In one of these, 40% increase in theactivity of the supply module results in less than 4% increase in ﬂux, while in nitesimal MMCA predicts more than 30% increase in ﬂux. In addition,it ... to increase the ﬂux by manipulating the activity of demand. The impossibility of increasing the ﬂux by changing the activity of a single module is due to an abrupt decrease of the control of ... consisted of varying the ATP ⁄ ADP ratio and measuring the supply and demand rates independently.The decrease in the ATP ⁄ ADP ratio was achieved byoverexpressing the hydrolytic part of the F1 domain...

Báo cáo khoa học: Sulfoquinovosylmonoacylglycerol inhibitory mode analysis of rat DNA polymerase b pdf

... nuc-leotide, single-strand and singly primed), and 0.15 nmol of the full-length pol b or the 8-kDa domain of pol b. Variousconcentrations of inhibitors were added to the bindingmixture, followed by incubation ... uniformly15N-labeled 8-kDa domain in the presence of increasingamounts of SQG. Of the 80 amides in residues 6–87 of the 8-kDa domain, 76 amides were assigned in the SQG complex using the CBCA(CO)NH and HNCACB spectra ... domain of pol b by a gelmobility shift assayWe investigated the interaction between the 8-kDadomain of pol b and SQMG in detail. The DNA bindingactivity of the 8-kDa domain was analyzed using...

Báo cáo khoa học: "Agar gel immunodiffusion analysis using baculovirus-expressed recombinant bovine leukemia virus envelope glycoprotein (gp51/gp30T-)" ppsx

... antibodies induced by the vaccine and BVDV in the BLV antigen preparation. The aim of this paper is therefore to describe the production of gp51 and partial gp30 by recombinant baculovirus in insect ... AGID using the indicated reference sera. The concentration of recombinant antigen was determined using a BCA protein assay kit (Pierce, USA), according to the manufacturer’s instructions, and ... encoded gp51 and the N-terminal region of gp30 lacking the C-terminal cytoplasmic domain and we demonstrated that BLV gp51/gp30T- was secreted from insect cells. The FLK cell line is still...

Báo cáo khoa học: "Dose volume histogram analysis of normal structures associated with accelerated partial breast irradiation delivered by high dose rate brachytherapy and comparison with whole breast external beam radiotherapy fields" potx

... conception and design, acquisition and analysis of data, manuscript writing. DOF, JH, RCwere involved in design, acquisition and analysis of data.AK, SM, PD were involved in conception, design and ... cc and 30 cc of thewhole heart volume (the D20 and D30 respectively) wasmeasured The volume of the heart receiving a dose of 5 Gy and higher, 10 Gy and higher and 20 Gy and higher (V5,V10 and ... of the heart and lungreceiving higher doses of radiation is significantly lowerusing the MammoSite catheter. The volume of heartreceiving low doses of radiation is significantly higherusing...

Tài liệu Báo cáo khoa học: Post-translational modiﬁcations of the linker histone variants and their association with cell mechanisms docx

... ubiquitinated in H1.2,H1.3 and H1.4 in human HeLa cells, but not in MCF7 cells. In mouse tissue, Lys116 of H1.1 and Lys46 of H1.2 and H1.3 in the spleen were the onlysites of ubiquitination. ... acetylation and methylation are also key modiﬁcations of the linkerhistones.Lysine and N-terminus acetylationsAcetylation of the Na-terminus involves the cotransla-tional cleavage of a methionine, ... acetyla-tion of the second residue of the N-terminal tail.Initial, non-MS work showed that only H1.0 in humancells and H5 in avian cells existed in forms that hadthe Na-terminus both acetylated and...

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