Báo cáo khoa học: " Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation" pps

... (2005), / 6 (1), 47–51 Immunization of mice with recombinant P27/30 protein confers protection against hard tick Haemaphysalis longicornis (Acari: Ixodidae) infestation Myung-Jo You* College of Veterinary ... (31.1%). Immunization of mice with rP27/30 protein confers protection against hard tick Haemaphysalis longicornis infestation....

Ngày tải lên: 07/08/2014, 18:21

... intensity of the band of unhydrolyzed actin was plotted against the time of incubation. Characterization of actin preparation quality Fluorescence parameters were used for estimation of nativity of ... may suppose that these two proteins can interact with each other. Analyzing the interaction of native actin with HSP25 purified from avian tissues and with recombinant p...

Ngày tải lên: 20/02/2014, 23:20

... size-distribution of oligomers obtained after hydrolysis of chitosan (F A ¼ 0.65) with ChiB to degrees of scission (a) varying from 0.03 to 0.37. Peaks are labelled with DP-values or with the sequence of the ... Size-distribution of oligomers after extended hydrolysis of various chitosans with ChiB. Chromatograms showing the size-distribution of oligomers obtained upon e...

Ngày tải lên: 07/03/2014, 16:20

... Phosphorylation of viral movement proteins – regulation of cell-to-cell traffick- ing. Trends Microbiol 9, 5–8. 45 Laemmli UK (1970) Cleavage of structural proteins during the assembly of the head of bacteriophage ... were coated with 5.0 lg of first protein (100 lL per well) at 37 °C for 2 h. The protein was diluted with 1· NaCl ⁄ P i (pH 7.4). The unadsorbed protein was...

Ngày tải lên: 15/03/2014, 00:20

... interaction of TATA box binding protein and TFIIA with DNA. Biochemistry 33, 7033–7040. 4760 E. Trotta et al. (Eur. J. Biochem. 270) Ó FEBS 2003 Interaction of DAPI with individual strands of trinucleotide ... compact structure of DAPI-bound ATT- template with respect to its free form, which is consistent with the previously reported lower mobility of the free monomeric ra...

Ngày tải lên: 16/03/2014, 23:20

... extracts, most of the endogenous UDE appears within the exclusion volume, at a very similar elution position to that of recombinant UDE complexed with RNA (Fig. 1). Treatment of pupal extract with RNase ... with primers specific for 200-nucleotide segments of either domain V of 23S rRNA (grey line), mRNA of UDE (black line), or mRNA of GAPDH (light grey line). C t values...

Ngày tải lên: 22/03/2014, 16:21

... that the interactions of these differ- ent proteins are likely to be indirect, depending on the presence of intact RNA as integral component of the RNP. Association of 42Sp50 with mRNA has not been reported ... that are formed with the corresponding signal sequences in the untranslated regions of the mRNAs and with a number of different so-called localization proteins. In th...

Ngày tải lên: 23/03/2014, 03:20

... to treatment with JH alone. Subordination of JH III signaling through USP to status of EcR activation There remains the question of why overexpression of EcR, leading to increased formation of EcR ... binding pocket of USP to activate transcription of the DR1JHECore. Overexpression of EcR in the trans- fected cells results in the loading of the DR1 enhancer of the DR1JHE...

Ngày tải lên: 23/03/2014, 13:20

... when NADH was used as cofactor. Fig. 2. Steady-state kinetics of recombinant and native GAPDH with varying concentrations of BPGA. (A) Recombinant GAPDH (final concentration of 1.5 · 10 )9 M , j) ... supplemented with 1 m M dithiothreitol, pH 7.9. The void volume of the column, determined with dextran blue, was 18 mL. Results Purification of recombinant C. reinhardtii GAPD...

Ngày tải lên: 23/03/2014, 20:22

... aminoacylation of tRNA with tryptophan showed that the best result, 96 pmol of Tyr per 1600 pmol of tRNA Phe , was obtained after 30 min of pressure at 6 kbar (Fig. 2). The yield of charging of crude ... aminoacylation of CoA, and in the acylation of mini helix of RNA [32]. The equilibrium of CoA acylation was shifted towards an aa-S-CoA formation [33]. In the case of...

Ngày tải lên: 30/03/2014, 10:20