Báo cáo khoa học: " Cap-independent protein translation is initially responsible for 4-(N-methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial pithelial cells" pot

... (2004), / 5 (4), 369–378 Cap-independent protein translation is initially responsible for 4-( N - methylnitrosamino)-1-(3-pyridyl)-butanone (NNK)-induced apoptosis in normal human bronchial epithelial cells Seo-Hyun ... cap- independent protein translation was responsible for early apoptosis. To understand the relative roles of cap-dependent and -independe...

Ngày tải lên: 07/08/2014, 18:20

... are distributed free of charge to not -for- profit institutions. Abbreviations hPrP, human prion protein; mPrP, mouse prion protein; PrP, prion protein; PrP C , cellular form of prion protein. FEBS ... prion protein (PrP) in its ‘cellular form’ (PrP C ) in healthy organisms is intimately related to susceptibility to transmissible spongiform encephal- opathies, such as scrapie i...

Ngày tải lên: 18/02/2014, 08:20

... recog- nizing the N-terminus [W1NT (A)] and C-terminus [W1CT (C)] of APLP1. Western blotting for GAPDH was included to check for equal protein loading (E). Lysates of a cell line overexpressing human ... removed, and the remaining brain was cut in half along the midline, snap frozen in liquid nitrogen, and stored at )80 °C until analysis. Levels of BACE1 protein in all of the...

Ngày tải lên: 15/03/2014, 10:20

... domain binding motif in their C-termini. PDZ domains have been established as sites for protein protein inter- action and play a central role in organizing diverse cell signaling assem- blies. In ... domains are modular protein protein interac- tion domains, which are amongst the most abundant protein interaction domains in organisms from bacteria to mammals, and have been imp...

Ngày tải lên: 18/02/2014, 13:20

... have resulted in their use for protein production in the laboratory. These systems have protein engineering appli- cations, such as the production of proteins containing unnatural amino acids and ... we generate integral membrane proteins within liposomes, and is there any way to integrate proteins into the lipid bilayer in the proper conforma- tion? Recent progress in answeri...

Ngày tải lên: 19/02/2014, 06:20

... detect Cyp4x1 in brain; quantitation of the Cyp4x1 protein in brain demonstrated  10 ng of Cyp4x1 protein mg )1 microsomal protein, showing that Cyp4x1 is a major brain P450. Immunohistochemical ... preincubated with purified recombinant Cyp4x1 protein, resulting in the ablation of detection of the 55-kDa protein in brain (Fig. 5C). This shows that the antisera specifically de...

Ngày tải lên: 19/02/2014, 07:20

... 1479 monomeric form of the protein [10]. This protein failed to assemble, form disks or interact with RNA in vitro [10]. On the basis of this result, we hypothesized that the N-terminus of the CP is involved ... well as for mapping domains of CPs involved in this process. The recombinant NLPs, with an average length of 50 nm, represent 20–30% of the total purified proteins. The rem...

Ngày tải lên: 07/03/2014, 05:20

... chains to increase the labelling efficiency. The nascent chain corresponding to the authentic preprotein comprised 101 amino acids, while the nascent chain corresponding to the Leu and Ala preproteins ... chain; ?, unknown cross-linking adducts; *, cross-linking adducts with E. coli L23; d, cross-linking adducts with E. coli Ffh; s, cross-linking adducts with B. subtilis Ffh; n , cross-linking...

Ngày tải lên: 16/03/2014, 22:20

... permeabilized for 2 min in 0.15% Triton X-100 in NaCl ⁄ P i , and washed again twice in NaCl ⁄ P i . Glass slides were next placed in a solution containing 1% ethanolamine in NaCl ⁄ P i for 20 min at ... an F-box protein, which acts as a recep- tor for target proteins. Skp1 is an adaptor between one of the variable F-box proteins and Cullin. At the G 1 ⁄ S transition, the F...

Ngày tải lên: 30/03/2014, 08:20

... Kv2.1 potassium channel in mammalian neurons [11]), protein kinases (e.g. MAP kinases [12,13] and Src family kinases [14]), adaptor proteins (e.g. SH2-domain containing leuko- cyte protein of ... nucleotide phosphodiesterase 3B; RS, arginine-serine repeats; SH2 domain, Src homology 2 domain; SP, serine–proline repeat; SRPK, serine-arginine- rich protein kinase; SRR, serine-rich region...

Ngày tải lên: 18/02/2014, 08:20