Báo cáo khoa học: "TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus" pot

... to RT-mix for synthesis of cDNA. The RT-mix consisted of 6 µ l of 5X Universal buffer, 3 µ l of 0.1 µ l M DDT, 1 µ l of 10 mM dNTP, 1 µ l of reverse primer (20 pM), 1 of Superscript reverse transcriptase ... Seoul 151-742, Korea One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japa...

Ngày tải lên: 07/08/2014, 18:20

Ngày tải lên: 07/08/2014, 18:21

... the intracellular growth of the pathogen in its host. This could explain the absence of the l-gulono- 1,4-lactone dehydrogenase reaction product in the M. tuberculosis cells grown in vitro. The ... l-gulono-1,4-lactone dehydrogenase of M. tuberculosis is a specific enzyme for the biosynthesis of L-AA. As far as we know, this is the first report of a specific biosynt...

Ngày tải lên: 07/03/2014, 12:20

Ngày tải lên: 07/08/2014, 20:23

... of adipogenic differentiatedhMSCs,alsointhecorner area s of the micro cavity (Figure 3f). The performed stu- dies verify the biocompatibility of the Si 3 N 4 membrane and the suitability of the ... sensitivity, the use of the miniaturized system for the detection of thres hold-dependent effects was tested. The comparison of the data obtained using a 96-...

Ngày tải lên: 21/06/2014, 00:20

... selective probe for metal ions detection. The anchoring to the surface of the CNTs was carried out by the reaction between the precursor and the carboxyl groups available on the surface of the support. ... suppress the fluorescence of MWNTs-GNQ. Thus Zn(II) considerably enhances the fluorescence of MWNTs-GNQ. To further characterize the performance of the...

Ngày tải lên: 22/06/2014, 01:20

... comparison of an actual waveform (the combined autopower spectra of each lead) to an ideal waveform (the cross-power spectrum of the two leads). This is expressed as the angle in degrees of the ... studies [11-14]. The overall sensitivity of 89.1% and specificity of 81.1% provided by the 3DMP device in the detection of hemodynamically relevant CAD confirms...

Ngày tải lên: 08/08/2014, 16:23

... to assess the performance of the prediction of sten osis independent of the prevalence of stenosis the positive and negative likelihood ratios (LR) were calculated [24]. A value of P < ... in the study group is in the range of what is reported in the literature from larger epidemiologic studies [14, 15, 16, 17]. The overal l sensitivity of 90.9% and specif...

Ngày tải lên: 08/08/2014, 17:20

... polymerases, and the solution of the crystal structure of Pol II shows that these two subunits are located close to each other at the surface of the polymerase [29,124] and participate in the tran- scription ... on the role of actin and ABPs in the modulation of the subcellular localization of transcriptional regulators. This review focuses on recent developments...

Ngày tải lên: 07/03/2014, 01:20

... assembly of for- oxymithine by a synthetase lacking all E-domains. The lack of sequence information for the S. nitrosporeus genome impeded the identification of a biosynthetic machinery governing foroxymithine ... to the identification of the second siderophore (data not shown). We therefore assume that either the extraction conditions were inadequate for the iso- la...

Ngày tải lên: 16/02/2014, 09:20