Báo cáo khoa học: "Molecular characterization of full-length

Báo cáo khoa học: "Molecular characterization of full-length genome of Japanese encephalitis virus (KV1899) isolated from pigs in Korea" pps

... Molecular characterization of Japanese encephalitis virus in Korea 201 mosquito pool in Korea in 1994 (98.5% nucleotide identity). Because Anyang strain is the current vaccine strain for swine, we ... Korea We have determined the complete nucleotide and deduced amino acid sequences of the Japanese encephalitis virus (JEV) strain KV1899, isolated from a fattening pi...

Ngày tải lên : 07/08/2014, 17:23

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Báo cáo khoa học: "Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pigs in Korea" pps

... unit *5-iodo-deoxyuridine. **Log TCID 50 /ml in Vero cells. ***Mouse brain antigen. Biophysical characterization of Japanese encephalitis virus (KV1899) isolated from pig in Korea 127 fluid using RNA isolation ... purified from brain homogenate of infected mice by ultracentrifugation. The purified JE virus from brain homogenate using ultracentrifuge revealed homo...

Ngày tải lên : 07/08/2014, 17:22

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Tài liệu Báo cáo khoa học: Molecular characterization and allergenic activity of Lyc e 2 (b-fructofuranosidase), a glycosylated allergen of tomato pdf

... isoform. Cloning of the cDNA of two isoforms of tomato b-fructofuranosidase and recombinant expression in E. coli For protein expression in E. coli, only the cDNA coding for the mature proteins ... USA) in pulse-liquid mode to determine the N-terminal partial sequence of the IgE-reactive protein. All chemicals were from Applied Biosystems. Cloning the cDNAs of two isoforms...

Ngày tải lên : 21/02/2014, 00:20

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Báo cáo khoa học: Molecular characterization and gene disruption of mouse lysosomal putative serine carboxypeptidase 1 ppt

... cassette, resulting in a Scpep1 ⁄ b-galactosidase ⁄ neo- mycin phosphotransferase fusion protein. The fusion protein is devoid of the C-terminal half of Scpep1, including two amino acids of the assumed catalytic ... result of the clinical relevance of soluble lysosomal proteins in LSDs and a notable number of LSD-like diseases of unknown etiology, there is a com- mon inter...

Ngày tải lên : 07/03/2014, 03:20

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Tài liệu Báo cáo khoa học: Molecular characterization of Arabidopsis thaliana PUF proteins – binding speciﬁcity and target candidates doc

... the binding of RNA-binding proteins to 3¢ UTR tran- scripts has been poorly described in plants. PUF proteins are a large family of RNA-binding proteins found in all eukaryotes. These proteins ... FEBS Binding of APUM to NRE To test the predictions regarding the RNA-binding speciﬁcities of the putative APUM proteins, we investi- gated the capacity of APUM to bind to the NRE sequ...

Ngày tải lên : 18/02/2014, 06:20

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Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... dpf in enterocytes of the pos- terior part of the intestine (Fig. 5M,Q,R). This last finding should be of interest because in terms of func- tion, the fish intestine is highly regionalized both in ... lumen [43,44]. Considering the importance of the intestinal barrier in the process of oral prion infection, this finding might help clarify the entry and routing of PrP Sc...

Ngày tải lên : 19/02/2014, 16:20

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Báo cáo khoa học: Molecular characterization of Osh6p, an oxysterol binding protein homolog in the yeast Saccharomyces cerevisiae pot

... protein–protein interaction. In this study, Osh6p was demonstrated to bind a pool of phosphatidylinositol phosphates (PtdInsP) including PtdIns(4)P, PtdIns(5)P, PtdIns(3,4)P 2 and PtdIns(3,5)P 2 with ... Faculty of Medicine, National University of Singapore, Republic of Singapore 2 Institute of Molecular and Cell Biology, A*STAR Biomedical Research Institutes, Singapore, Republi...

Ngày tải lên : 07/03/2014, 21:20

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Báo cáo khoa học: Molecular characterization of H2O2-forming NADH oxidases from Archaeoglobus fulgidus potx

... might have remained in the Nox assay. The decrease in NADH in the ﬁrst assay was related to the amount of H 2 O 2 found in the second assay. Protein was determined according to Bradford [18] using the ... 3,3¢-dimethoxybenzidine as electron donor. In the NoxA-1 assay between 71% and 95% of the amount Fig. 3. SDS/PAGE of extracts of recombinant E. coli containing NoxA-1, NoxB...

Ngày tải lên : 08/03/2014, 02:21

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Báo cáo khoa học: Molecular characterization of a novel nuclear transglutaminase that is expressed during starﬁsh embryogenesis ppt

... : 26 of the original volume using Centricon-10 (Amicon). Proteins were determined by the modiﬁed method of alkaline copper (Lowry) protein assay [23] using BSA as the standard. Transglutaminase ... localization of TG has been reported in the studies on tissue TG [8,9]. A nuclear transport protein, importin-a3, has been shown to be involved in the active transport of tissue TG into...

Ngày tải lên : 08/03/2014, 10:20

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Báo cáo khoa học: Molecular characterization of a blood-induced serine carboxypeptidase from the ixodid tick Haemaphysalis longicornis docx

... (1999) Vaccination with cathepsin L proteinases and with leucine aminopeptidase induces high levels of protection against fascioliasis in sheep. Infect Immun 67, 1954–1961. 3 Williamson AL, Brindley ... activity was deﬁned as lmol of leucine, alanine or tyrosine released per min per mg of protein. The kinetic parameters k cat and K m were obtained from Hanes– Woolf plots. To examine...

Ngày tải lên : 16/03/2014, 10:20

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