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Báo cáo khoa học: "Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp Paratuberculosis" pot

Báo cáo khoa học:

Báo cáo khoa học: "Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. Paratuberculosis" pot

... USA). Antibody response to five recombinant antigens and sero -diagnosis with 35 kDa for M. avium subsp. paratuberculosis113Dialysis of proteins and removal of endotoxinsThe recombinant proteins ... by variations in the stage of infection. In the attempt to increase sensitivity, severalmodifications have been tried including using a combination of antigens or a single recombinant antigen ... sera in related to shedding level confirmed by fecal culture and IS900 PCRTable 2. Comparison of cut-off point, sensitivity and specificity of individual recombinant antigen in ELISA assayAntigens...
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Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt

Báo cáo khoa học: HIP/PAP, a C-type lectin overexpressed in hepatocellular carcinoma, binds the RIIa regulatory subunit of cAMP-dependent protein kinase and alters the cAMP-dependent protein kinase signalling ppt

... a175 amino acid p rotein containing only one carbohydrate-binding d omain (CRD) linked to an N-terminal sequence,part of which is cleaved during its maturation and secretion[5]. In humans, ... blotting for HIP/PAP usingpolyclonal antibody anti-HIP/PAP and for phosphorylatedserine using polyclonal anti-phosphoserine (Zymed Labor-atories).Cell fractionationHIP/PAP expressing and control ... PAP1(referred to as Reg 2) is an important neurotrophic factor for motor neurones in vitro and in vivo in the rat [11,12]. In liver recombinant HIP/PAP has been shown to promotethe adhesion of rat hepatocytes...
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báo cáo khoa học: "Sentinel lymph node biopsy for high-risk cutaneous squamous cell carcinoma: clinical experience and review of literature" ppsx

... shown in Table 1.Upon pathologic examination with conventional H&Estaining, there was no evidence of metastatic carcinoma in any of the submitted lymph nodes. Immunostainingwas performed ... dif-ferentiation, perineural invasion, recurrent lesions, sites of prior radiotherapy or chronic inflammation, immuno-compromised states, and genetic disorders includingalbinism and xeroderma pigmentosum. ... lesions of the head and facedid not undergo i ntraoperative blue dye injection. Asmall skin incision was made overlying the SLN locationas determined by preoperative lymphoscintigraphy and intraoperative...
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Báo cáo khoa học: Aldo-keto reductase from Helicobacter pylori – role in adaptation to growth at acid pH doc

Báo cáo khoa học: Aldo-keto reductase from Helicobacter pylori – role in adaptation to growth at acid pH doc

... lgÆmL)1).Production of HpAKR was initiated by addition of isopropylthio-b-d-galactoside to a final concentration of 1 mm, and this was followed by incubation at room temperature, to minimize inclusion body formation. ... reduction of aldehydes and ketones to the corre-sponding alcohol product, and thus serve to removepotentially cytotoxic and mutagenic aldehydes fromcells [14]. In view of the potential aldehyde detoxifying ... of Clinical Medicine and Institute of Molecular Medicine, Trinity College Dublin, IrelandHelicobacter pylori is one of the most common humanpathogens, and has been strongly linked with chronicgastritis,...
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báo cáo khoa học: " The Rx for Change database: a first-in-class tool for optimal prescribing and medicines use" ppt

... 5:89http://www.implementationscience.com/content/5/1/89Page 6 of 9We organize the r eviews of interventions directed to professionals and the delivery of care using the EPOCtaxonomy of interventions. This taxonomy groups inter-vent ions into five ... S: Development of a taxonomy of interventions directed at consumers to promote evidence -based prescribing and medicines use: a tool for evidence -based decision-making (Poster session 3: Safe and ... 9taxonomy of consumer directed interventions [9,10].Examples of interventions include the provision of infor-mation or education and behaviour change support. Weprovide definitions of each interventio...
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báo cáo khoa học: " Implementing the LifeSkills Training drug prevention program: factors related to implementation fidelity" pps

... coordinator), and instituting at least some minimal form of internalmonitoring that provides corrective feedback to teachers and administrators regarding implementation.Competing interestsThe ... LSTlessons be a minimum of 50 minutes.A key aspect of the LST curriculum is variation in instruc-tors' teaching techniques that includes didactic instruc-tion, discussion, demonstration, and ... required for all LST instructors and localcoordinators, and was encouraged for school administra-tors and other support staff. (See Additional File 1 for more details regarding LST training workshops...
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Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

Tài liệu Báo cáo khoa học: Comparative studies of endonuclease I from cold-adapted Vibrio salmonicida and mesophilic Vibrio cholerae docx

... stained with Simply Blue Safe Stain(Invitrogen) according to the manufacturer’s protocol. Theprotein concentration was determined using Bio-Rad Pro-tein Assay based on the method of Bradford ... incubation at 98 °C in a PCR machine for 3 min, and then kept on ice. Approximately 300 ng of the varioussubstrates was mixed with 30 ng enzyme in a total volume of 20 lL containing 1 mm MgCl2 and ... passage of ions, and the peri-plasmic proteins are, like the extracellular proteins,constantly exposed to the salinity of the surroundingwater. Knowledge on cold adaptation is in manycases based...
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Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

Tài liệu Báo cáo khoa học: Comparative studies on the functional roles of N- and C-terminal regions of molluskan and vertebrate troponin-I pdf

... troponin T. ATPase activation and binding to tropo-nin I and troponin C. J Biol Chem 273, 10594–10601.31 Pearlstone JR & Smillie LB (1995) Evidence for two-sitebinding of troponin I inhibitory ... themaintenance of structural integrity of the troponincomplex in the relaxed state.Troponin-tropomyosin based regulation exhibits twocomponents [32]: inhibition and removal of inhibition in ... TnC-binding site and the C-domain of TnC accompanyingCa2+binding to site IV of TnC. In vertebrate tropo-nin, the activation may be a result of the interactionbetween the inhibitory region and...
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Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx

Báo cáo khoa học: Comparative structure analysis of proteinase inhibitors from the desert locust, Schistocerca gregaria pptx

... inhibitor 2: expression , k inetic analysis of in -hibition w ith alph a-ch ymotrypsin and wild-type an d m utan tsubtilisin BPN ¢, and protein engineering to investigate inhibitoryspeci®city and ... studies of internal dynamics of serine proteinase protein inhibitors: binding region mobilities of intact and r eactive-site hydrolized Cucur bita maxima trypsininhibitor (CMT I)-II of the squash ... i s a potent trypsin inhibitor, and SGTI inhib its both proteinases weakly. Interestingly, SGTIis a much better inhibito r of insect proteinases than of t hemammalian ones used in common assays....
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Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

Báo cáo khoa học: Comparative biochemical and functional studies of family I soluble inorganic pyrophosphatases from photosynthetic bacteria potx

... pres-ence of trimers in solution. All determinations of nativemolecular masses reported here were performed withexcess of Mg2+ in solution to allow the interactionsinvolved in hexamer formation. ... UÆmg)1protein and recovery levels of 20–30%. In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed onlyone protein band of 20–22 kDa (Figs 1 and 2; supple-mentary ... pRGP and pRGCT) containing ppa ORFs and the correspondingupstream regions were sequenced to ensure that ppa geneswere cloned in the opposite orientation to the lacZ vec-tor’s promoter and that...
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