Báo cáo khoa học: "Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp Paratuberculosis" pot

... USA). Antibody response to five recombinant antigens and sero -diagnosis with 35 kDa for M. avium subsp. paratuberculosis 113 Dialysis of proteins and removal of endotoxins The recombinant proteins ... by variations in the stage of infection. In the attempt to increase sensitivity, several modifications have been tried including using a combination...

Ngày tải lên: 07/08/2014, 17:22

... a 175 amino acid p rotein containing only one carbohydrate- binding d omain (CRD) linked to an N-terminal sequence, part of which is cleaved during its maturation and secretion [5]. In humans, ... blotting for HIP/PAP using polyclonal antibody anti-HIP/PAP and for phosphorylated serine using polyclonal anti-phosphoserine (Zymed Labor- atories). Cell fractionation HIP/PAP expre...

Ngày tải lên: 23/03/2014, 13:20

... shown in Table 1. Upon pathologic examination with conventional H&E staining, there was no evidence of metastatic carcinoma in any of the submitted lymph nodes. Immunostaining was performed ... dif- ferentiation, perineural invasion, recurrent lesions, sites of prior radiotherapy or chronic inflammation, immuno- compromised states, and genetic disorders including albinism and...

Ngày tải lên: 09/08/2014, 02:20

... lgÆmL )1 ). Production of HpAKR was initiated by addition of isopropyl thio-b-d-galactoside to a final concentration of 1 mm, and this was followed by incubation at room temperature, to minimize inclusion body formation. ... reduction of aldehydes and ketones to the corre- sponding alcohol product, and thus serve to remove potentially cytotoxic and mutagenic aldehydes f...

Ngày tải lên: 30/03/2014, 04:20

... 5:89 http://www.implementationscience.com/content/5/1/89 Page 6 of 9 We organize the r eviews of interventions directed to professionals and the delivery of care using the EPOC taxonomy of interventions. This taxonomy groups inter- vent ions into five ... S: Development of a taxonomy of interventions directed at consumers to promote evidence -based prescribing and med...

Ngày tải lên: 10/08/2014, 10:23

... coordinator), and instituting at least some minimal form of internal monitoring that provides corrective feedback to teachers and administrators regarding implementation. Competing interests The ... LST lessons be a minimum of 50 minutes. A key aspect of the LST curriculum is variation in instruc- tors' teaching techniques that includes didactic instruc- tion, discussion, d...

Ngày tải lên: 11/08/2014, 05:22

... stained with Simply Blue Safe Stain (Invitrogen) according to the manufacturer’s protocol. The protein concentration was determined using Bio-Rad Pro- tein Assay based on the method of Bradford ... incubation at 98 °C in a PCR machine for 3 min, and then kept on ice. Approximately 300 ng of the various substrates was mixed with 30 ng enzyme in a total volume of 20 lL con...

Ngày tải lên: 19/02/2014, 05:20

... troponin T. ATPase activation and binding to tropo- nin I and troponin C. J Biol Chem 273, 10594–10601. 31 Pearlstone JR & Smillie LB (1995) Evidence for two-site binding of troponin I inhibitory ... the maintenance of structural integrity of the troponin complex in the relaxed state. Troponin-tropomyosin based regulation exhibits two components [32]: inhibition and re...

Ngày tải lên: 20/02/2014, 01:20

... inhibitor 2: expression , k inetic analysis of in - hibition w ith alph a-ch ymotrypsin and wild-type an d m utan t subtilisin BPN ¢, and protein engineering to investigate inhibitory speci®city and ... studies of internal dynamics of serine proteinase protein inhibitors: binding region mobilities of intact and r eactive-site hydrolized Cucur bita maxima trypsin inhibitor...

Ngày tải lên: 08/03/2014, 10:20

... pres- ence of trimers in solution. All determinations of native molecular masses reported here were performed with excess of Mg 2+ in solution to allow the interactions involved in hexamer formation. ... UÆmg )1 protein and recovery levels of 20– 30%. In all cases, the analysis by SDS ⁄ PAGE and native PAGE of purified preparations showed only one protein band of 20...

Ngày tải lên: 16/03/2014, 11:20