... region of the pBIND vector into the pAc5.1 ⁄ V5-His Avector. Another control vector pAcMet was prepared bysubcloning the full ORF of Met into the pAc5.1 ⁄ V5-His Avector. An empty pAc5.1 ... receptors: many actors in search of a plot. Cell83, 851–857.48 Swanson HI, Chan WK & Bradfield CA (1995) DNAbinding specificities and pairing rules of the Ah recep-tor, ARNT, and SIM proteins. ... thecorresponding amounts of 3H-labeled JH III and processed in parallel with the programmed lysates, and the countsobtained were taken as nonspecific binding. Specific bindingwas obtained by subtracting...