Báo cáo khoa học: "Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System" ppsx

... Effects of Protein Source and Energy Substrates on the In Vitro Development of Bovine Embryos in a Two-step Culture System 75 a two-step culture system. Development to blastocyst in medium containing ... always comprised of a single formulation for all stages [20]. Many have investigated the in vitro culture of bovine embryos, but...

Ngày tải lên: 07/08/2014, 17:22

... MAPs are again a mixture of proteins containing predominantly MAP2 and tau with traces of MAP1, MAP4, etc. [33]. We checked the Fig. 1. Insulin and ADH aggregation in the presence of MTP. (A) Aggregation ... patches in MAP2 and tau, it is noted that they contain 18.6 and 12.7% acidic amino acids, respectively, indicating both of them are predominantly basic in nat...

Ngày tải lên: 23/03/2014, 12:20

... Mizoguchi A, Ishizaki H, Nagasawa H, Kataoka H, Isogai A, Tamura S, Suzuki A, Fujino M & Kitada C (1987) A monoclonal antibody against a synthetic frag- ment of bombyxin (4K-prothoracicotropic ... mori, as in many other holo- metabolous insects, the basal external structure of adult appendages such as wings, legs and antennae have already been built at the time of pup...

Ngày tải lên: 18/02/2014, 13:20

... can also maintain the edge-to-face interaction, provi- ding the stabilization effect of the heme binding. In the case of the Phe35fiLeu mutant, however, substitution of leucine for phenylalanine ... concentration of protein could cause denaturation of the apo -protein during the long assay time, all of the heme transfer reactions were monitored only by the UV...

Ngày tải lên: 08/03/2014, 10:20

... using a FACSCalibur (Becton-Dickinson, San Jose, CA, USA). Surface biotinylation of proteins To analyze the stability of CD38 on the plasma membrane of the different Ba/F3 mutants, labeling of ... USA). Protein concentration was determined with the BCA protein assay (Pierce). The catalytic activity of CD38 in Ba/F3 cell homogenates was determined by HPLC using t...

Ngày tải lên: 23/03/2014, 12:20

... some preparations of the enzyme after activation could be traced to the presence of a contaminating phosphatase activity in these preparations. The addition of fresh IMP to these preparations restored ... rates of the unactivated enzyme. Surprisingly, acti- vation was observed only upon incubation with PRPP and hypoxanthine, and not with guanine and xanthine, the o...

Ngày tải lên: 16/03/2014, 18:20

... zinc atom in the protease domain and a phos- phate group in the helicase domain are also visible. The interface region between the helicase and the protease domains is framed and shown in detail in ... [8–13]. Nevertheless, we are interested in determining the functional importance of the helicase domain for the activity and inhibition of the protease...

Ngày tải lên: 16/03/2014, 06:20

... reading frame. Amino acid numbering starts from the N-terminal amino acid. Resistance level determined after a 24 h incubation on LB agar. Strain Position of mutation in rpsL Amino acid replacement Resistance ... MBq, Amer- sham), and was incubated at 37 °C for 15 min. The background value was obtained from the reaction in the absence of poly(U). After the incubation,...

Ngày tải lên: 30/03/2014, 13:20

... that induce apoptosis, including UV- and c-irradiation, heat shock, protein synthesis inhibitors, DNA-damaging agents and the proinflam- matory cytokines, are potent activators of JNK. Several anti-neoplastic ... 91–118. 19 Hibi M, Lin A, Smeal T, Minden A & Karin M (1993) Identification of an oncoprotein- and UV-responsive protein kinase that binds and potentiates the...

Ngày tải lên: 18/02/2014, 13:20

... types and organ- isms (Table 1). Each of these kinases modifies its target protein and is itself a target for the upstream kinase. Because the concentration of kinases and their target proteins are ... reduced and the upstream kinases of MAPK and MAPKK are only slightly activated and can sequester only limited fractions of MAPK and MAPKK. Discussion The functi...

Ngày tải lên: 19/02/2014, 07:20