Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ" potx

Báo cáo khoa học: Identiﬁcation of tyrosine-phosphorylation sites in the nuclear membrane protein emerin pot

... attachedto the lamina by integral membrane proteins of the INM. The INM proteins form complexes, transientlyor stably, with lamins, chromatin proteins and a vari-ety of regulatory proteins, including ... improving the search for phosphopeptidescontaining phosphotyrosine, we identiﬁed one of the well-deﬁned integral INM proteins. Emerin is a type-IIintegral membrane protein of 34 kDa, which is inser-ted ... phosphotyrosineimmunosignal may be misleading, since the afﬁnity of phosphotyrosine-speciﬁc antibodies is always in u-enced by amino acid residues surrounding the phos-photyrosine. Finally, emerin in...

Báo cáo khoa học: "Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ" potx

... Characterization of the Lectin-binding Sites in the Equine Vomeronasal Organ 17DiscussionThis is the first study to examine the binding of DBA,SBA, Isolectin B4, WGA, PNA, and UEA-I in the vomeron-asal ... sensoryepithelium [15]. Nevertheless, the carbohydrate-specificity of the sensory epithelium of the equine VNO remains unresolved. The carbohydrate (lectin-binding) moieties of glycoproteinsand ... were stained for lectins.Histochemical Characterization of the Lectin-binding Sites in the Equine Vomeronasal OrganJee-young Lee, Tae-young Kang, Yong-duk Lee and Tae-kyun Shin*Department of Veterinary...

Báo cáo khoa học: Kinetic characterization of methionine c-lyases from the enteric protozoan parasite Entamoeba histolytica against physiological substrates and triﬂuoromethionine, a promising lead compound against amoebiasis ppt

... 1.6-foldincrease in the Kmand a 58% decrease in the kcatforCys. This implies that the hydroxyl group of Tyr108 of EhMGL1 is actively involved in the b-elimination andc-elimination of the C–S ... primers having BamHI andXbaI sites for ‘nested PCR’, using the ﬁrst PCR product astemplate. To make use of the BamHI site in the vector and the XbaI site in the EhMGL1 gene, the product of the nes-ted ... 116% or 42.4% in the present study). The discrepancy in the kinetic constants of EhMGL1 wasprobably attributable to the heterogeneity of the EhMGL1 preparation in the previous study. The VmaxABFig....

Báo cáo khoa học: Biochemical characterization of recombinant dihydroorotate dehydrogenase from the opportunistic pathogenic yeast Candida albicans pot

... understand the mode of bindingand interference with enzyme catalysis.As the inactivation of any enzyme involved in ametabolic chain will render the whole chain inoperat-ive, the inactivation of any ... of the six proteinsinvolved in pyrimidine de novo synthesis should result in the same profound effect on the pyrimidine nuc-leotide pools in C. albicans. However, in mammaliancell lines, the ... oxidation of the substrate DHO with the quinone cosub-strate was coupled to the reduction of the chromogen 2,6-di-chloroindophenol. The Km of DHO was determined byvarying the concentration of DHO...

Tài liệu Báo cáo khoa học: Molecular defect of isovaleryl-CoA dehydrogenase in the skunk mutant of silkworm, Bombyx mori ppt

... cDNAsequences using the RACE method in both strainsrevealed the presence of a single point mutation in 1337 nucleotides of BmIVD. In the sku mutant, the 1127th guanine from the start codon was ... acts in the third step of leucine degradation and the sku mutant accumulates branched-chain amino acids in haemolymph, thismutant may be useful in the investigation of unique branched-chain aminoacid ... surface of the monomer, pointing toward FAD or its neigh-bouring monomer in the tetramer. By contrast, the side chain of the mutated residue (Val376) pointstoward the inside of the helix–loop–helix...

Tài liệu Báo cáo khoa học: Molecular aspects of rheumatoid arthritis: chemokines in the joints of patients pdf

... chemokines stimu-lated mainly by in ammatory cytokines, including IL-1b, TNF-a andIFN-c. (B) Chemokines expressed in the joint recruit leukocytes into the joints. In addition to functioning in ... regulated mainly by TNF-a. The role of chemokines in RA pathogenesis The chemokines listed above are implicated in RApathogenesis via the recruitment and retention of leu-kocytes in the joints. In addition ... subclasses include the CC chemokines(where the cysteines are neighboring) and the CXCchemokines (where the cysteines are separated by oneamino acid). The CXC chemokines mainly act on neu-trophils...

Tài liệu Báo cáo khoa học: Electrostatic role of aromatic ring stacking in the pH-sensitive modulation of a chymotrypsin-type serine protease, Achromobacter protease I pdf

... dependence of the pH-activity curve of API The pH-activity proﬁles depending on NaCl concentrationwere determined as part of the investigation into the shielding from solvent of the electrostatic interactionbetween ... position. In the struc-tural deviation, the solvent ASA of the side-chain of His210increased with the decrease in size of the side-chain atresidue 169 (Table 1 and Fig. 3A). However, the ASAs of Asp113 ... size of the side-chain at residue 169 and the pK2showed a clear linear relationship (Fig. 3B). The pK2increased as the size of the residue at position 169 increased. The effect of the size of...

Báo cáo khoa học: Membrane binding of SRP pathway components in the halophilic archaea Haloferax volcanii docx

... beenreported that in addition to the cluster of positive chargesfound at the start of the N-terminal FtsY A domain, asecond region contained within the NG domain of the protein participates in FtsY membrane ... encodingfor a C-terminally polyhistidine-tagged version of the protein. Given that the presence of a His6-tag at the C-terminus of the E. coli FtsY NG domain did notinterfere with determination ... unexpected, having been previ-ously reported in the case of E. coli FtsY [11] andattributed to the amino acid composition of the acidicdomain (A domain) of the protein [25,34]. Moreover, the aberrant...

Báo cáo khoa học: Proteomic characterization of lipid raft proteins in amyotrophic lateral sclerosis mouse spinal cord pot

... with the intensity of the internal standard in the same MS scan, and then compared with the normalizedintensity of the same peptide in another sample to calculate the ratio of the peptides between ... abundances in WT and G93A lipid raft samples,as well as the alteration ratios, are listed in Table 2. The remaining 113 proteins, including actin, tubulin,coﬁlin, and SOD1, showed either no changes in ... microdomains of the plasma membrane enriched in cholesterol and sphingo-lipids. These rafts act as platforms for the organizationand interaction of proteins involved in multiple func-tions, including...

Báo cáo khoa học: " Driving Forces of Forest Cover Dynamics in the Ca River Basin in Vietnam" ppt

... specifically, the objectives of the study are (i) to estimate the rates of forest cover changes in the upper Ca River Basin during the period 1998 - 2003 and (ii) to determine the main socio-economic ... the accuracy assessment of the land cover/land use map later. 33Driving Forces of Forest Cover Dynamics in the Ca River Basin in Vietnam The results of land cover mapping are shown in ... and diminished the terrain effect on the surface reflectance in order to gain a better land cover mapping. Moreover, experience gained by working with satellite images gathered during the region’s...

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