Báo cáo khoa học: "Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" pdf

... is to examine the expression of Embryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune EncephalomyelitisTae-kyun Shin*, Yong-duk ... is in partEmbryonic Intermediate Filaments, Nestin and Vimentin, Expression in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis11Fig. 1.Immunohistochemical staining of ... cells and multipotent progenitor cells. In the present study, the expression patterns of nestin and vimentin in the spinal cords of rats w ith experimental autoimmune encephalomyelitis (EAE) and the...

Báo cáo khoa học: "Immunohistochemical Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis" docx

... ether anesthesia, and the spinal cords were removed and frozen in a deep freezer (-70 C) forprotein analysis. Pieces of the spinal cords were processedfor paraffin embedding after fixation in ... of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis283Mice overexpressing Bcl-2 in their neurons are resistantto myelin oligodendrocyte glycoprotein (MOG)-induced experimental ... Localization of Bcl-2 in the Spinal Cords of Rats with Experimental Autoimmune Encephalomyelitis281CFA-immunized group on the same day (day 9) (Fig. 1, lane2). Surprisingly, Bcl-2 expression declined...

Báo cáo khoa học: Relationship between functional activity and protein stability in the presence of all classes of stabilizing osmolytes ppt

... [21–25], and DGD° values in the presence of these osmolytes are given in Table 1. How-ever, DGD° values of RNase-A in the presence of alanine, serine, lysine, b-alanine, taurine and dimethyl-glycine ... advances in understanding the effect of osmolytes on protein stability, folding and the activity of proteins and enzymes, the relationshipbetween protein stabilization by osmolytes and its con-sequent ... hydration of the protein domain is the driving force of protein stabiliza-tion or folding [3,4]. Furthermore, the effect of osmolytes on the functional activity of an enzyme hasalso been investigated...

Tài liệu Báo cáo khoa học: Collagen I regulates matrix metalloproteinase-2 activation in osteosarcoma cells independent of S100A4 pdf

... depending on the time and localization of their expression. The N-terminal part of TIMPs is involved in binding to the active site of MMPs and hence prevents their action, whereas the C-terminal ... °C in culture wells with or without monomeric col-lagen. Neither of these two conditions altered the rela-tive amount of the activated forms, indicating thatbinding of the pro (72 kDa) or intermediate ... acystein residue in the prodomain and Zn2+ in the active site of the catalytic domain. Two major types of endogenous inhibitors regulate the activity of MMPs;a2-macroglobulin and four tissue inhibitors...

Tài liệu Báo cáo khoa học: Molecular characterization, phylogenetic relationships, and developmental expression patterns of prion genes in zebraﬁsh (Danio rerio) doc

... part of the intestine (Fig. 5M,Q,R). This lastﬁnding should be of interest because in terms of func-tion, the ﬁsh intestine is highly regionalized both in the larva and in the adult [38]. The ... (h), and intestinal epithelium (ie) (M, insert) of the posterior intestine(pi). In the CNS, the labeled divisions are the telencephalon (t), mesencephalon (m) including the optic tectum (ot), the ... 2004 FEBS and in speciﬁc areas outside the CNS of the developingzebraﬁsh. The prp1 and prp2-expressing sites in the developing ﬁsh system could correlate with the time and sites of PrP1 and PrP2...

Tài liệu Báo cáo khoa học: "A Multimodal Interface for Access to Content in the Home" pdf

... understanding of system capa-bilities. Of the remaining 66% of inputs (2166) which were within the functionality of the system, 68% were in grammar. On the within functionality portion of the ... completing the same two sets of scenarios and then freely interacting with each system. For counterbalancing purposes, half of the subjects used the tablet and then the re-mote control and the other ... and pen input, includ-ing both pointing and handwriting (Figure 1). Our application task also differs, focusing on search and browsing of a large database of movies-on-demand and supporting...

Báo cáo khoa học: Evidence for two different electron transfer pathways in the same enzyme, nitrate reductase A from Escherichia coli potx

... fumarate reduction; the use of the quinol-binding site inhibitor HOQNO shows that the inhibitor blocks binding of MQH2at the QPsite [27].Study of the reduction and oxidation of the cytochromehas ... four kineticphases in the reduction of the hemes by menaquinol.According to the quinone type used, the two hemes of the cytochrome are probably involved in the transfer of electrons from the ... analogues of physiological quinones, usingmenadione as an analogue of menaquinone and duro-quinone as an analogue of ubiquinone, and comparing the results with those obtained with dithionite. The...

Báo cáo khoa học: X-ray crystallography, CD and kinetic studies revealed the essence of the abnormal behaviors of the cytochrome b5 Phe35ﬁTyr mutant pdf

... phenylalanineat position 35 does not destroy the aromatic interactions and can also maintain the edge-to-face interaction, provi-ding the stabilization effect of the heme binding. In the case of the ... normallyretained by the prosthetic heme group; core 2 comprisesmainly b-sheets. These two cores are well maintained in the apo-form of the protein [43] and so are especially interestingfor the study of ... chainconformation of this residue. The shift of the Ca atom of Tyr35 of the Phe35ﬁTyr mutant from that of Phe35 of the wild-type cyt b5is 0.21 A˚, within the error limit. The sidechain of...

Báo cáo khoa học: Structural origins for selectivity and speciﬁcity in an engineered bacterial repressor–inducer pair pdf

... shown in grey and blue). The stereo represen-tation shows that the binding position of the ligands and the orientations of the side chains lining the binding sites are highly conserved in the ... conformations of the residues lining the bindingsite (Table 2). In an initial comparison of the ligandpositions of tetracycline, dox and atc in TetR, theseligands superimpose with an average rmsd of ... comprises the dimer interface. The two effector-binding sites present in the dimer are identical and, because the binding sites are located within the proteininterface, each binding site is lined...

Báo cáo khoa học: A new rice zinc-ﬁnger protein binds to the O2S box of the a-amylase gene promoter pptx

... pLGD17-265UP1wasusedasthebaitwithaninsertion of three copies of the O2S domain at the 5¢ end of the CYC1 mini-promoter region. Following transfor-mation of the URA-marked plasmid pLGD-265 UP1containing the ... protein thatcontains a zinc-ﬁnger. Our experimental data indicate thatRAMY protein binds speciﬁcally to the O2S elemen t. Wehave also determined the importance of the amino acidswithin the binding ... speciﬁcity of binding target sequence [25]. However, we only detected onezinc-ﬁnger domain in RAMY, perhaps because the differentstructure of the zinc ﬁnger motif in RAMY in uences the binding domain....

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