... normalizing against products of b-actin. The x-axis indicates the time-periods of LPS incubation and the relative expression of the carp IL-10 gene on the y-axis. Controls for 0, 1, 3 and 6 h of incubation ... expression and its role in the regulation of other pro -in ammatory genes, such as TNF and IFN, in fish, need to be conducted. In conclusion, the...
Ngày tải lên: 20/02/2014, 02:21
... introduction of aggregation-disrupting amino acid substitutions in the aggregation- prone ⁄ amyloidogenic short sequence regions suggests the possibility of fine-tuning and controlling the solu- bility of ... residue of the protein, equal to the probability of backbone–back- bone hydrogen bond formation observed for this type of residue; then, the obtained values ar...
Ngày tải lên: 06/03/2014, 00:20
Tài liệu Báo cáo khoa học: Biochemical characterization and inhibitor discovery of shikimate dehydrogenase from Helicobacter pylori docx
... dose–response equation of the origin software (OriginLab, Northampton, MA, USA). Afterwards, inhibitor modality was determined by measur- ing the effects of inhibitor concentrations on the enzymatic activity ... rate of 0.2 mLÆmin )1 . The peptide fraction was detected by PDA (TSP UV6000) and directly introduced on- line into ESI source. The operating condition was optimi...
Ngày tải lên: 19/02/2014, 05:20
Tài liệu Báo cáo khoa học: Functional expression and mutational analysis of flavonol synthase from Citrus unshiu pptx
... structural integrity of the active FLS, point mutations were initiated aiming at the substitution of glycine residues by alanine or proline and the exchange of proline by glycine. Glycine residues ... gene, and the essence of most of the conserved amino acids has been further substantiated by site-directed mutagenesis of FHT [7,9] and by documentation of ligand b...
Ngày tải lên: 21/02/2014, 03:20
Tài liệu Báo cáo khoa học: Helix mobility and recognition function of the rat thyroid transcription factor 1 homeodomain – hints from 15N-NMR relaxation studies pdf
... remarkable as inferred from the NOE data, have little effect on the value of the Æs mi æ estimate, and adds confidence to the assumption of isotropic motion adopted by the equation of the relax- ation rate ... [21]. The fit was obtained by linear regression, and only the corresponding J(0)–J(x N ) correlation plot is shown in Fig. 4. The localization of th...
Ngày tải lên: 18/02/2014, 16:20
Tài liệu Báo cáo khoa học: Molecular modeling and functional characterization of the monomeric primase–polymerase domain from the Sulfolobus solfataricus plasmid pIT3 doc
... with reaction mixtures containing only the indicated (d)NTPs in addition to the DNA template and the protein, whereas lane 0 contains a control reaction without protein. Analysis of the pIT3 prim–pol ... both the R186 residue in the Rep245 domain and K300, its counterpart in Pfu-primase, were contained in a loop that is plausibly involved in DNA recognition and b...
Ngày tải lên: 18/02/2014, 18:20
Báo cáo khoa học: Global shape and pH stability of ovorubin, an oligomeric protein from the eggs of Pomacea canaliculata pot
... [13]. Except for the detailed studies on crustacyanin, the lobster carapace carotenoprotein, there is little infor- mation on the structure and stability of this interesting group of proteins, and there ... giving the best fit to the scattering intensity. The resulting dummy atom model represents the shape of the scattering particle. To increase the reliability...
Ngày tải lên: 07/03/2014, 06:20
Báo cáo khoa học: Purification and structural analysis of the novel glycoprotein allergen Cyn d 24, a pathogenesis-related protein PR-1, from Bermuda grass pollen pot
... numbers on the left of the figure indicate the positions of the deduced amino acid sequence. N-terminal segment determined by protein sequencing is underlined. (B) Comparison of the amino acid sequence ... proteins including isoforms ⁄ degraded protein products and around 65 of these are IgE-binding proteins [19]. Only a few of these proteins have been purified and cha...
Ngày tải lên: 07/03/2014, 12:20
Báo cáo khoa học: Purification and structural study of the b form of human cAMP-dependent protein kinase inhibitor pdf
... & Zhao, S. (2000) Cloning and mapping of human PKIB and PKIG, and comparison of tissue expression patterns of three members of the protein kinase inhibitor family, including PKIA. Biochem. J. ... amide I¢ component bands, determined by band fitting of the absorbance spectrum of Fig. 4A, are shown in Fig. 6 and Table 2. From Table 2, the sum of individual ami...
Ngày tải lên: 07/03/2014, 15:20
Báo cáo khoa học: Solution structure and backbone dynamics of the XPC-binding domain of the human DNA repair protein hHR23B docx
... ubiquitin-like (UbL) domain, the XPC-binding domain, and two ubiquitin-associated domains (UBA1 and UBA2) [9,10]. The UbL domain has a high bind- ing affinity for polyubiquitin binding site 2 of the human ... because of secondary effects from the divergent positioning of the flexible N-terminal region (275–283), the boundary and size of HT1 were variable; in contra...
Ngày tải lên: 07/03/2014, 17:20