Báo cáo khoa học: "Localization of Porcine Reproductive and Respiratory " pptx

... components. N-glycosylation sites of bovine ZPA and porcine ZPB [10,11] and ZPC [13] in the ZP domains are shown by filled black boxes. Arrows and C indicate the linkage sites of sperm-ligand carbohydrate chains and positions ... Asn83, Asn191 and Asn527 of ZPA are glycosylated. Determination of the number of N-glycosylation sites We then investigated the number of N-gl...

Ngày tải lên: 21/02/2014, 03:20

... assays with increasing amounts of DNA fragments encoding cyt b 5 and monitored the formation of androstadienol and DHEA. As shown in Fig. 5 the stimulation of DHEA and androstadienol production from ... transformation of preg into androstadienol, a precursor in the biosynthesis of Fig. 5. Influence of increasing concentrations of cyt b 5 on the relative formation of andro...

Ngày tải lên: 08/03/2014, 08:20

... cut-out band and immunoblotting indicated bands at 220 kDa, 125 kDa, 97 kDa, 68 kDa and 45 kDa as in previous investigations, which were supportive of a precurser, two forms of polymalatase and proteolytic ... extracted and assayed for polymalate. Molecular mass standards were BSA, ferritin and their oligomers. The samples were: 0.5 lg of purified polymalate; a mixture of pur...

Ngày tải lên: 17/03/2014, 10:20

... acute stimulation of PKC and PKA. Stimulation of PKC and PKA has n o detectable effect on the e xpression of TauT in NIH3T3 fibroblasts (data not shown) and most probably does not involve modulation of the ... M iyamoto and coworkers [5] that TauCl is involved in oxidation of IjB which prevents phosphorylation of IjB and a ctivation o f NF-jB. Thus, the effect of longte...

Ngày tải lên: 30/03/2014, 15:20

... of InsP 6 to PhyAsr (Table 1). Given the absence of structural changes as a function of ionic strength and ligand binding, the effect of ionic strength on catalytic activity is probably not of ... move- ment of the P-loop in PhyAsr is due to oxidation of the catalytic cysteine, we oxidized crystals of PhyAsr Table 1. Effect of ionic strength on the hydrolysis of InsP 6 b...

Ngày tải lên: 18/02/2014, 18:20

... Sedla ´ c ˇ ek 3 and Jindr ˇ ich Has ˇ ek 1 1 Institute of Macromolecular Chemistry, 2 Institute of Organic Chemistry and Biochemistry and 3 Institute of Molecular Genetics, Academy of Sciences of the ... C c atoms of Phe53 and Phe153). In the case of Ôwide tunnelÕ, the diameter of t he effective view through the solvent channel is 12.4 A ˚ (measured a s a distance of...

Ngày tải lên: 19/02/2014, 16:20

... action of EPA and DHA (Eur. J. Biochem. 271) 4471 Modulation of cyclin D1 synthesis and hyperphosphorylation of Rb by n-3 and n-6 PUFAs Induction of cyclin D1 is one of the e arliest effects of mitogenic ... contribute to the modu- lation of egr-1 gene expression (Fig. 4B). Effects of n-3 and n-6 PUFAs on the synthesis of caveolin-1 and caveolin-3 and on the loca...

Ngày tải lên: 19/02/2014, 16:20

... to learn thou- sands of Chinese characters. The official agents in Taiwan and China, respectively, chose 5401 and 3755 characters as important basic characters in national standards. Consequently, ... e.g., “賣” and “讀” are pronounced as /mai4/ and /du2/, respectively. In contrast, the pro- nunciations of “匋”, “淘”, “陶”, and “啕 ” are /tao2/. Pronunciations of specifi...

Ngày tải lên: 19/02/2014, 19:20

... consisting of the CSN, the 26S pro- teasome and cullin-based Ub ligases. Permanent expression of a chimerical subunit (Flag-CSN2-Rpn6) consisting of the N-terminal 343 amino acids of CSN2 and of the ... role of the PCI domain of CSN2 for complex formation and consequences of permanently overex- pressed CSN2 for the stability of p53 and c-Jun. The CSN interacts direct...

Ngày tải lên: 20/02/2014, 01:20

... T m of lysozyme was 77.3 °C in the absence of additives. The addition of 100 m M polyamines and Arg slightly increased the T m of lysozyme by  1 °Cand0.5°C, respectively, whereas addition of ... Tadayuki Imanaka 3 and Masahiro Takagi 1 1 School of Materials Science, Japan Advanced Institute of Science and Technology, Ishikawa, Japan; 2 Department of Bioscience, School...

Ngày tải lên: 20/02/2014, 02:21